RMHMAG-84K

MILLIPLEX^® Rat Metabolic Hormone Magnetic Bead Panel - Metabolism Multiplex Assay

The analytes available for this multiplex kit are: Amylin (Active), C-Peptide 2, Ghrelin (Active), GIP (Total), GLP-1 (Active), Glucagon, IL-6, Insulin, Leptin, MCP-1, PP, PYY, TNF-α.

• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

Properties

• Quality Level: 200
• species reactivity: rat
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 103%; (Amylin (Active)); accuracy: 96%; (GLP-1 (Active)); accuracy: 96%; (IL-6); accuracy: 96%; (MCP-1); accuracy: 98%; (TNFα); accuracy: 99%; (C-Peptide 2); accuracy: 99%; (Insulin); sensitivity: 1-62 pg/mL; standard curve range: 14-10,000 pg/mL; (Glucagon & TNFα); standard curve range: 2.7-2,000 pg/mL; (GIP (Total)); standard curve range: 41-30,000 pg/mL; (Amylin (Active) & GLP-1 (Active)); standard curve range: 69-50,000 pg/mL; (C-Peptide 2, IL-6, Insulin, Leptin & MCP-1); standard curve range: 7-5,000 pg/mL; (Ghrelin (Active), PP & PYY (Total))
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

Metabolic syndrome is a cluster of conditions, including increased blood pressure, elevated insulin levels, abnormal cholesterol levels and excess body fat around the waist. Key features of metabolic syndrome include insulin resistance, glucose intolerance, hypertension, dyslipidemia, and central obesity.

MILLIPLEX^® Rat Metabolic Panel is a 13-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/cell lysate and culture supernatant samples and serum or plasma samples: Amylin (active), C-peptide 2, Active Ghrelin, GIP, GLP-1, Glucagon, IL-6, Insulin, Leptin, MCP-1, PP, PYY, and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

Specificity

Cross Reactivty
No significant cross-reactivity with other hormones tested.

Application

• Analytes: Amylin (Active), C-Peptide 2, Ghrelin (Active), GIP (Total), GLP-1 (Active), Glucagon, IL-6, Insulin, Leptin, MCP-1 (CCL2), Pancreatic Polypeptide (PP), PYY (Total), TNFα
• Recommended Sample Type: Rat serum, plasma, cell/tissue culture supernatants and lysates.
• NOTE: For specific analytes protease inhibitors should be added upon blood collection: DPPIV for GLP-1 (Active); Protease Inhibitor Cocktail for Amylin (Active); Aprotinin for Glucagon; Serine Protease Inhibitor for Ghrelin (Active)
• Recommended Sample Dilution: 25 μL per well of neat serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
• Assay Run Time: Overnight (18-20 hours) at 2-8°C
• Research Category: Metabolism
• Research Subcategory: Metabolic Disorders, Obesity, Endocrine

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS06,GHS09
• signalword: Danger
• hcodes: H302 + H332,H311,H317,H411
• pcodes: P261 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1
• supp_hazards: EUH032
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects