PC138
Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb
liquid, Calbiochem®
Synonym(s):
Anti-Nuclear Factor ΚB
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
rabbit
Quality Level
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Rabbit polyclonal antibody. Recognizes the ~65 kDa NF-κB p65 (RelA) protein.
Recognizes the ~65 kDa NF-κB p65 (RelA) protein in Daudi cells.
This Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb is validated for use in ELISA, Gel Shift, Immunoblotting, Immunoprecipitation for the detection of NF-κB (p65, RelA) (Ab-2).
Immunogen
Human
a synthetic peptide corresponding to amino acids near the N-terminal domain of human NF-κB p65 (RelA), conjugated to KLH
Application
ELISA (see comments)
Gel Shift (see comments)
Immunoblotting (1:500, chemiluminescence)
Immunoprecipitation (see comments)
Gel Shift (see comments)
Immunoblotting (1:500, chemiluminescence)
Immunoprecipitation (see comments)
Warning
Toxicity: Standard Handling (A)
Physical form
In 5 mM sodium phosphate buffer, pH 7.4.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Daudi cells
Daudi cells
Other Notes
Kolberg, R., 1997. J. NIH Research9, 29.
Van Antwerp, D.J. et al. 1996. Science274, 787.
Beg, A.A., et al. 1995. Nature376, 167.
Baeuerle, P.A., and Henkel, T., 1994. Ann. Rev. Immunol.12, 141.
Wulczyn, F.G., et al. 1992. Nature358, 597.
Van Antwerp, D.J. et al. 1996. Science274, 787.
Beg, A.A., et al. 1995. Nature376, 167.
Baeuerle, P.A., and Henkel, T., 1994. Ann. Rev. Immunol.12, 141.
Wulczyn, F.G., et al. 1992. Nature358, 597.
This antibody has also been reported to work for ELISA, gel shift, and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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