antibody form
purified antibody
clone
polyclonal
form
liquid
contains
0.03% thimerosal as preservative
species reactivity
rat, human
isotype
IgG
General description
Neurotrophins are a family of proteins that interact with neurotrophin receptors and provide various survival signals in nervous tissue. p75 neurotrophin receptor (p75NTR) is also known as the low-affinity neurotrophin receptor, while TrkA, TrkB, and TrkC are the high affinity receptors. p75NTR is a member of the tumor necrosis factor receptor family and is common to all neurotrophins. Similar to the high affinity receptors, p75NTR will bind the neurotrophins, but unlike the Trk proteins, it has no cytoplasmic tyrosine kinase domain. The roles of this receptor are controversial, as it may also be involved in either promoting or downregulating the response to the neurotrophins. It may also function to increase the affinity of the trk receptors for their respective neurotrophins or it may bind neurotrophins and prevent them from binding to the high affinity receptors. p75NTR does not have a catalytic intracellular tyrosine kinase domain, but it is still capable of mediating the neurotrophin signals.
Purified rabbit polyclonal antibody. Recognizes the ~75 kDa p75 neurotrophin receptor protein.
Recognizes the ~75 kDa neurotrophin receptor (p75NTR) protein in cultured mouse fibroblasts stably transfected with human p75NTR. Also recognizes additional lower MW proteins that are cleavage products of p75NTR.
This Anti-p75 Neurotrophin Receptor Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry for the detection of p75 Neurotrophin Receptor.
Immunogen
a recombinant protein consisting of amino acids 273-425 of rat p75NTR
Application
Immunoblotting (1-2 μg/ml)
Immunocytochemistry (1-5 μg/ml)
Immunocytochemistry (1-5 μg/ml)
Physical form
In PBS.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Mouse fibroblasts stably transfected with human p75NTR
Mouse fibroblasts stably transfected with human p75NTR
Other Notes
The immunogen sequence comprises the C-terminal intracellular domain. Antibody should be titrated for optimal results in individual systems.
Storage Class
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
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Kevin C Kanning et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 23(13), 5425-5436 (2003-07-05)
The 75 kDa neurotrophin receptor (p75NTR) and two neurotrophin receptor homologs (NRH1, NRH2) constitute a subfamily of the nerve growth factor/tumor necrosis factor receptor superfamily. NRH1 coexists with p75NTR in fish, amphibians, and birds but is absent in mammals, whereas
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