MIT1000
Mitochondria/Cytosol Fractionation Kit
This Mitochondria/Cytosol Fractionation Kit provides reagents for quick & efficient isolation of intact mitochondria from cultured cells.
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.75
Recommended Products
Quality Level
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
technique(s)
cell culture | mammalian: suitable
application(s)
sample preparation
shipped in
dry ice
General description
Mitochondria, sometimes described as the “power plants” of the cell, are sites where most of the energy production in eukaryotic cells takes place. The synthesis of most of the adenosine triphosphate (ATP) occurs in these double membraned organelles that are found in living cells. ATP production by the mitochondria is done by the process of respiration, which uses oxygen to generate energy. This is a very efficient process for using food energy to make ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signaling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria also regulate crucial apoptosis signaling pathways. The number of mitochondria in a cell varies widely by organism and tissue type. Many cells have only a few mitochondria, whereas others can contain several thousand.
Millipore’s Mitochondria/Cytosol Fractionation Kit provides reagents for quick and efficient isolation of intact mitochondria from cultured cells. This kit allows mitochondrial isolation by using a convenient table top microcentrifuge, and can be used to separate an enriched mitochondrial fraction (heavy membrane fraction) from cytosolic fraction (light membrane fraction). Such separation is useful for studying apoptosis and signaling pathways between the two fractions, by Western blotting or ELISA.
The kit contains sufficient materials for the analysis of 50 samples.
Millipore’s Mitochondria/Cytosol Fractionation Kit provides reagents for quick and efficient isolation of intact mitochondria from cultured cells. This kit allows mitochondrial isolation by using a convenient table top microcentrifuge, and can be used to separate an enriched mitochondrial fraction (heavy membrane fraction) from cytosolic fraction (light membrane fraction). Such separation is useful for studying apoptosis and signaling pathways between the two fractions, by Western blotting or ELISA.
The kit contains sufficient materials for the analysis of 50 samples.
Application
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Components
Isotonic Mitochondrial Buffer (CS204255): 50 mL
Mitochondrial Lysis Buffer (CS204257): 5 mL
Protease inhibitor cocktail (CS204253): 1 mL
Anti-Bcl2 (05-729-25UG): 25 μg
Anti-GAPDH (CS204254): 25 μg
Mitochondrial Lysis Buffer (CS204257): 5 mL
Protease inhibitor cocktail (CS204253): 1 mL
Anti-Bcl2 (05-729-25UG): 25 μg
Anti-GAPDH (CS204254): 25 μg
Storage and Stability
All components should be store at -20°C for up to one year from date of receipt.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Eye Irrit. 2 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
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