MABT859M

Anti-CDCP1 (CD318) Antibody, clone 8A2

clone 8A2, from mouse

• Synonym(s): CUB domain-containing protein 1, Membrane glycoprotein gp140, Subtractive immunization M plus HEp3-associated 135 kDa protein, SIMA135, Transmembrane and associated with src kinases, CD318
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 8A2, monoclonal
• species reactivity: human
• packaging: antibody small pack of 25 μg
• technique(s): flow cytometry: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_073753.3
• UniProt accession no.: Q9H5V8
• target post-translational modification: unmodified
• Gene Information: human ... CDCP1(64866)

Description

General description

CUB domain-containing protein 1 (UniProt: Q9H5V8; also known as Membrane glycoprotein gp140, Subtractive immunization M plus HEp3-associated 135 kDa protein, SIMA135, Transmembrane and associated with Src kinases, CD318) is encoded by the CDCP1 (also known as TRASK) gene (Gene ID: 64866) in human. CUB domain-containing protein 1 is a single-pass membrane protein that is synthesized with a 29 amino acid signal peptide, which is subsequently cleaved to generate the mature form. A soluble form of CUB domain-containing protein 1 has also been reported that is produced by proteolytic cleavage at the cell surface. CUB domain-containing protein 1 is highly expressed in mitotic cells with low expression during interphase. It is detected at highest levels in skeletal muscle and colon. Lower expression is reported in kidney, small intestine, placenta, and lung. It is also reported in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells. CUB domain-containing protein 1 levels are up-regulated in a number of human tumor cell lines and in colorectal cancer, breast carcinoma and lung cancer. It is reported to be involved in cell adhesion and cell matrix association and may play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation.

Specificity

Clone 8A2 detects CUB domain-containing protein 1 (CDCP1) in human cells. it targets an epitope within the extracellular domain.

Immunogen

His-tagged recombinant fragment corresponding to 638 amino acids from the extracellular domain of human CUB domain-containing protein 1.

Application

Anti-CDCP1 (CD318), clone 8A2, Cat. No. MABT859, is a mouse monoclonal antibody that detects CUB domain-containing protein 1 (CDCP1) and has been tested for use Flow Cytometry.

Flow Cytometry Analysis: A representative lot detected CDCP1 (CD318) in one million MDA and MDA KO cells (Courtesy of the Laboratory of Feng Lin, Ph.D., Cleveland Clinic, Lerner Research Institute, Cleveland OH USA).

Quality

Evaluated by Flow Cytometry in DLD1 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected CDCP1 (CD318) in one million DLD1 cells.

Target description

92.93 kDa calculated.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1