OSTERIX, or SP7, is a zinc finger-containing, osteoblast-specific transcription factor that is distinctly expressed in all developing bones, and is a key requirement for the differentiation of pre-osteoblasts into fully functional osteoblasts. While research on the actual functional mechanisms of OSTERIX is still on-going, current findings indicate that OSTERIX expression is induced by BMP-2 and Dlx5, and operates downstream of RUNX2, supporting its role in mesenchymal stem cell differentiation. Further studies also indicate that the OSTERIX transcription factor may also play a role in negatively regulating the Wnt/β-catenin signaling pathways and the proliferation of osteroblast cell types during embryonic osteogenesis.
Immunogen
GST-tagged recombinant protein corresponding to human Osterix.
Application
Detect the Osterix (SP7) protein using this Anti-Osterix (SP7), clone 6B12.1 validated for use in WB & IHC.
Immunohistochemistry Analysis: A 1:200 dilution from a representative lot detected Isocitrate dehydrogenase in rat tooth tissue. Nuclear staining may be observed in epithelial cells in some tissues.
Research Category Cell Structure
Research Sub Category Cytoskeletal Signaling
Quality
Evaluated by Western Blotting in SW1353 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Osterix in 10 µg of SW1353 cell lysate.
Target description
~45 kDa observed. An uncharacterized band may appear at ~160 kDa in some lysates.
Physical form
Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control SW1353 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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