MABT177

Anti-Syndecan-1 Antibody, clone 11G2.1

clone 11G2.1, from mouse

• Synonym(s): Syndecan-1, CD138, SYND1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 11G2.1, monoclonal
• species reactivity: human
• technique(s): flow cytometry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001006947
• UniProt accession no.: P18827
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... SDC1(6382)

Description

General description

Syndecan-1 (UniProt P18827; also known as CD138, SYND1) is encoded by the SDC1 (also known as SDC) gene (Gene ID 6382) in human. Syndecan-1 belongs to a family of transmembrane heparan sulfate proteoglycans (HSPGs), members of which also include syndecan-2 (fibroglycan, SDC2), syndecan-3 (N-syndecan, SDC3), and syndecan-4 (amphiglycan, ryudocan, SDC4). Syndecan-1 is produced with a signal peptide (a.a. 1-22), the removal of which yields the mature protein with a large extracellular region (a.a. 23-254), a transmembrane segment (a.a. 255-275), and a cytoplasmic tail (a.a. 276-310). Syndecan-1 is modified at its extracellular domain on a.a. 45 and 47 with O-linked heparan sulfate (HS) that plays an important role in mediating its interaction with HS-binding protein, including growth factors (e.g. bFGF/FGF2, PDGF, TGF-β, and VEGF) and extracellular matrix proteins (e.g. collagens, fibronectin, thrombospondin, and tenascin). Syndecan-1 thus funtions as a coreceptor for growth factor receptors and acts as an anchor connecting the extracellular matrix to the intracellular cytoskeleton. Syndecan extracellular domain can be proteolytically cleaved off at a juxtamembrane site and upregulated shedding of the ectodomain occurs in response to growth factors, chemokines, heparanase, microbial toxins, insulin, and cellular stress. SDC1 deregulation contributes to cancer progression by promoting angiogenesis and cancer cell proliferation, metastasis, and invasion.

Specificity

Clone 11G2.1 recognizes an extracellular epitope.

Immunogen

Epitope: Extracellular domain.

GST-tagged recombinant human Syndecan-1 extracellular domain fragment.

Application

Anti-Syndecan-1 Antibody, clone 11G2.1 is an antibody against Syndecan-1 for use in Western Blotting, Flow Cytometry.

Flow Cytometry Analysis: 1.0 µg from a representative lot detected Syndecan-1 in one million Raji and Ramos cells.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Quality

Evaluated by Western Blotting of human syndecan-1 GST fusion protein.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected 0.05 µg of a GST-tagged human syndecan-1 extracellular domain fragment.

Target description

30.00 kDa calculated. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable