A disintegrin and metalloproteinase with thrombospondin motifs 5, ADAM-TS 5, ADAM-TS5, ADAMTS-5, EC 3.4.24.-, A disintegrin and metalloproteinase with thrombospondin motifs 11, ADAM-TS 11, ADAMTS-11, ADMP-2, Aggrecanase-2
The ADAMTS (A Disintegrin and Metalloprotease with Thrombospondin motifs) family of secreted proteases comprises at least 20 members in mammals, and several members in invertebrates. ADAMTS members resemble the membrane-bound ADAM family of proteases in the presence of a prodomain, a catalytic domain and a disintegrin-like domain. However, ADAMTS members lack a transmembrane domain, and instead contain varying numbers of thrombospondin type I modules. ADAMTS-5, along with ADAMTS-4, cleaves aggrecan between Glu373 and Ala374, a physiological cleavage site in arthritic cartilage. In addition, expression of ADAMTS-5 is elevated in human chondrocytes treated with inflammatory cytokine interleukin-1, but is constitutively expressed in bovine cartilage explants. 70 and 53 kD forms of ADAMTS-5 have been shown to be present at varying ratios in fibroblast-like synoviocytes and extracts of rheumatoid arthritis, osteoarthritis and normal synovial tissue.
Immunogen
Epitope: C-terminus
GST-tagged recombinant protein corresponding to the C-terminus of human ADAMTS5.
Application
Detect ADAMTS5 using this Anti-ADAMTS5 Antibody, clone 3A2.1 validated for use in IHC (Paraffin).
Research Category Cell Structure
Research Sub Category Cytoskeletal Signaling
Quality
Evaluated by Immunohistochemistry in human thalamus tissue.
Imunnohistochemistry Analysis: A 1:50 dilution of this antibody detected ADAMTS5 in human thalamus tissue.
Target description
101.7 kDa calculated
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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