MABT124

Anti-Leukemia-associated RhoGEF/LARG Antibody, clone 13D2.1

clone 13D2.1, from mouse

• Synonym(s): Rho guanine nucleotide exchange factor 12, Leukemia-associated RhoGEF
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 13D2.1, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable; western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_056128
• UniProt accession no.: Q9NZN5
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ARHGEF12(23365)

Description

General description

The protein named Leukemia-associated RhoGEF (LARG) or alternatively known as Leukemia-associated RhoGEF protein, and encoded by the gene ARHGEF12/KIAA0382/LARG, plays a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) proteins. Leukemia-associated RhoGEF (LARG) acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. Additionally, Leukemia-associated RhoGEF (LARG) interacts with RHOA, PLXNB1 and PLXNB2, and it interacts with IGF1R beta subunit and GCSAM proteins. Leukemia-associated RhoGEF (LARG) is localized to the cytoplasm and membrane. The protein translocates to the membrane upon stimulation. Leukemia-associated RhoGEF (LARG) widely expressed with isoform specific isoforms being found in certain tissues only, such as isoform 2 is primarily found in the intestine and testis.

Immunogen

Epitope: RGSL Domain

GST-tagged recombinant protein corresponding to the RGSL domain of human Leukemia-associated RhoGEF/LARG.

Application

Anti-Leukemia-associated RhoGEF/LARG Antibody, clone 13D2.1 is a highly specific mouse monoclonal antibody, that targets Leukemia-associated RhoGEF & has been tested in western blotting & ICC.

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Leukemia-associated RhoGEF/LARG in HepG2 and HeLa cells.

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

Quality

Evaluated by Western Blotting in HUVEC cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Leukemia-associated RhoGEF/LARG in 200 µg of HUVEC cell lysate.

Target description

~ 173 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable