MABS777

Anti-FADS1, clone EPR6898, Rabbit Monoclonal Antibody

clone EPR6898, from rabbit

• Synonym(s): Fatty acid desaturase 1, Delta(5) fatty acid desaturase, D5D, Delta(5) desaturase, Delta-5 desaturase, FADS1, FADSD5
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: unpurified
• antibody product type: primary antibodies
• clone: EPR6898, monoclonal
• species reactivity: human
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• UniProt accession no.: O60427
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... FADS1(3992)

Description

General description

Fatty acid desaturase 1, also known as Delta (5 fatty acid desaturase or D5D or Delta (5 desaturase or Delta-5 desaturase, and encoded by the gene name FADS1 or FADSD5, is a member of the fatty acid desaturase (FADS) family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs.

Specificity

Dissociation constant (KD) is 7.20 x 10-11 M

Immunogen

Synthetic peptide corresponding to human FADS1.

Application

Anti-FADS1, clone EPR6898, Rabbit Monoclonal Antibody is an antibody against FADS1 for use in Western Blotting, Immunohistochemistry, Flow Cytometry, Immunocytochemistry.

Immunohistochemistry Analysis: A 1:100-250 dilution from a representative lot detected FADS1 in human heart tissue.

Flow Cytometry Analysis: A 1:100-1,000 dilution from a representative lot detected FADS1 in HepG2 cells.

Immunocytochemistry Analysis: A 1:100-250 dilution from a representative lot detected FADS1 in A549 cells.

Produced in collaboration with Epitomics

Research Category
Signaling

Research Sub Category
Lipid Signaling

Quality

Evaluated by Western Blotting in human fetal lung, human fetal liver, human fetal brain, and HepG2 lysates.

Western Blotting Analysis: A 1:1,000-10,000 dilution of this antibody detected FADS1 in human fetal lung, human fetal liver, human fetal brain, and HepG2 lysates.

Target description

~52 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Unpurified

Rabbit monoclonal in buffer containing PBS with up to 0.1% sodium azide, 0.05% BSA, and 50% glycerol.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2