MABS513
Anti-CYP7B1 Antibody, clone M17P3F2
clone M17P3F2, from mouse
Synonym(s):
25-hydroxycholesterol 7-alpha-hydroxylase, EC:1.14.13.100, Cytochrome P450 7B1, Oxysterol 7-alpha-hydroxylase
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
M17P3F2, monoclonal
species reactivity
human
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1λ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... CYP7B1(9420)
General description
25-hydroxycholesterol 7-alpha-hydroxylase (UniProt: O75881; also known as EC:1.14.13.100, Cytochrome P450 7B1, Oxysterol 7-alpha-hydroxylase) is encoded by the CYP7B1 gene (Gene ID: 9420) in human. CYP7B1 is an enzyme involved in bile acid biosynthesis and shares 40% sequence identity to human cholesterol 7a-hydroxylase (CYP7A1). CYP7B1 localizes to the endoplasmic reticulum membrane and is expressed in brain, testis, ovary, prostate, liver, colon, kidney, and small intestine. It catalyzes the rate-limiting step in the conversion of cholesterol to bile acids. The classic pathway of bile acid synthesis is initiated by cholesterol 7alpha-hydroxylase in the liver and is tightly regulated by bile acid feedback, hormones, and diurnal rhythm. In contrast, the pathway initiated with sterol 27-hydroxylase is located mainly in extrahepatic tissues. An "alternative" pathway of bile acid synthesis begins with 27-hydroxylation of cholesterol by 27-hydroxylase (CYP27), followed by 7alpha-hydroxylation by oxysterol 7alpha-hydroxylase (CYP7B1). Mutations in CYP7B1 have been associated with Spastic paraplegia 5A, an autosomal recessive disorder that is characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Disorders in CYP7B1 also cause congenital bile acid synthesis defect 3 (CBAS3) that leads to severe cholestasis, cirrhosis, and liver synthetic failure.
Specificity
Clone M17P3F2 detects CYP7B1 in human cerebral cortex, testis, pancreatic cancer, and colon cancer.
Immunogen
Ovalbumin-conjugated linear peptide corresponding to 9 amino acids from the C-terminal end of human 25-hydroxycholesterol 7-alpha-hydroxylase (CYP7B1).
Application
Detect CYP7B1 using this mouse monoclonal Anti-CYP7B1 Antibody, clone M17P3F2, Cat. No. MABS513, used in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected CYP7B1 in human cerebral cortex and human pancreatic cancer tissue.
Immunohistochemistry Analysis: A representative lot detected CYP7B1 in in human liver tissue. (Courtesy of Ayham Alnabulsi, Ph.D., Vertebrate Antibodies Ltd. United Kingdom).
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected CYP7B1 in human liver tissue lysate.
Immunohistochemistry Analysis: A representative lot detected CYP7B1 in in human liver tissue. (Courtesy of Ayham Alnabulsi, Ph.D., Vertebrate Antibodies Ltd. United Kingdom).
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected CYP7B1 in human liver tissue lysate.
Research Category
Signaling
Signaling
Quality
Evaluated by Immunohistochemistry in human testis tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected CYP7B1 in human testis tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected CYP7B1 in human testis tissue.
Target description
58.26 kDa calculated.
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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