Indoleamine 2,3-dioxygenase (IDO) is an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO may be involved in the suppression of the immune response to tumours and blocking the IDO pathway may be a potential target for immunotherapy.
Specificity
This antibody does not cross react with Ido2.
Immunogen
Recombinant protein corresponding to mouse Ido1.
Application
Anti-Ido1, clone 8G11 Antibody is an antibody against Ido1 for use in Western Blotting, Flow Cytometry.
Western Blotting Analysis: A representative lot detected Ido1 in splenic dendric cells from WT or KO mouse tissue lysate (Oberdan Leo, Universite Libre de Bruxelles). Flow Cytometry Analysis: A representative lot detected Ido1 in splenic dendric cells from mouse (Oberdan Leo, Universite Libre de Bruxelles).
Quality
Evaluated by Western Blotting in Ido1-Flag tag transfected HEK293T cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ido1 in 10 µg of Ido1-Flag tag transfected HEK293T cell lysate.
Target description
~44 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physical form
Format: Purified
Other Notes
Concentration: Please refer to lot specific datasheet.
Not finding the right product?
Try our Product Selector Tool.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Conventional dendritic cells (cDCs), cDC1 and cDC2, act both to initiate immunity and maintain self-tolerance. The tryptophan metabolic enzyme indoleamine 2,3-dioxygenase 1 (IDO1) is used by cDCs in maintaining tolerance, but its role in different subsets remains unclear. At homeostasis
Questions
Reviews
★★★★★ No rating value
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
