MABS475

Anti-GRP-78 Antibody, clone C107

clone C107, from mouse

• Synonym(s): 78 kDa glucose-regulated protein, GRP-78, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: C107, monoclonal
• species reactivity: mouse, human
• technique(s): flow cytometry: suitable; western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_005338
• UniProt accession no.: P11021
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... HSPA5(3309)

Description

General description

The gene HSPA5 encodes a protein named GRP-78 (78 kDa glucose-regulated protein) also known as BiP (immunoglobulin heavy chain-binding protein) or heat shock 70 kDa protein 5. While originally discovered as one of set of genes that were induced upon cellular starvation and stress, HSPA5 encoded GRP-78 is now known to be an important member of the heat-shock family of proteins. The HSPA5 encoded GRP-78 protein appears to play a role in helping in the assembly of multimeric protein complexes in the endoplasmic reticulum (ER). Cellular localization is in the endoplasmic reticulum lumen and appears as cytoplasmic-like staining. The HSPA5 encoded protein is an essential component of the translocation processes within the endoplasmic reticulum and also functions in the retrograde transport across the ER membrane of incorrectly folded proteins destined to be degraded by the ubiquitin proteasome system. The HSPA5 encoded protein, while widely expressed in all cells types under normal conditions, is particularly induced under conditions of cellular stress, heat, or toxins and conditions which lead to the accumulation of unfolded proteins in the ER.

Immunogen

Recombinant protein corresponding to human GRP-78.

Application

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

This Anti-GRP-78 Antibody, clone C107 is validated for use in western blotting, flow cytometry & Inhibits activity/Function for the detection of GRP-78.

Western Blotting Analysis: 1 µg/mL from a representative lot detected GRP-78 in 10 µg of HeLa cell lysate.

Flow Cytometry Analysis: A representative lot from an independent laboratory detected GRP-78 in B16F1 cells from tumor bearing mice (de Ridder, G. G., et al. (2012). Melanoma Res. 22(3):225-235.).

Activity Assay Analysis: A representative lot from an independent laboratory reduces tumor growth in mice with B16F1 melanoma cells (de Ridder, G. G., et al. (2012). Melanoma Res. 22(3):225-235.).

Quality

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected GRP-78 in 10 µg of HepG2 cell lysate.

Target description

~72 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable