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MABS330

Sigma-Aldrich

Anti-SFRP2 Antibody, clone 8H1

clone 8H1, from rat

Synonym(s):

Secreted frizzled-related protein 2, FRP-2, sFRP-2, Secreted apoptosis-related protein 1, SARP-1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

8H1, monoclonal

species reactivity

mouse, horse

species reactivity (predicted by homology)

equine (based on 100% sequence homology), human (immunogen homology)

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SFRP2(6423)

General description

Secreted frizzled-related proteins (SFRPs) may play a critical role in carcinogenesis by inhibiting the Wnt/beta-catenin signaling pathway. Secreted frizzled-related protein 2 (SFRP2) may be useful to further investigate mammary tumorigenesis as its expression increases with tumor progression in canine mammary gland tumors. SFRP2 in mouse embryos has been found to cause severe shortening of the thoracic region. Studies have shown that this protein is the essential stem cell paracrine factor that is thought to regulate myocardial survival and repair damage in the aftermath of an ischemic injury.

Immunogen

Recombinant protein corresponding to human SFRP2.

Application

Detect SFRP using this rat monoclonal antibody, Anti-SFRP2 Antibody, clone 8H1 validated for use in western blotting & IHC.
Research Category
Signaling
Research Sub Category
Developmental Signaling
Western Blotting Analysis: A representative lot from an independent laboratory detected SFRP2 in RMG cell lysate and in ERU retinal tissue lysate (Hauck, S. M., et al. (2012). Journal of Proteomics. 75:4545-4554).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected SFRP2 in equine retinal tissue (Hauck, S. M., et al. (2012). Journal of Proteomics. 75:4545-4554).

Quality

Evaluated by Western Blotting in mouse eye tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected SFRP2 in 10 µg of mouse eye tissue lysate.

Target description

~ 33 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse eye tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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