MABS1727

Anti-CYB5R3 Antibody, clone 1D9.2

clone 1D9.2, from mouse

• Synonym(s): NADH-cytochrome b5 reductase 3, B5R, Cytochrome b5 reductase, Diaphorase-1
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1D9.2, monoclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_000389
• UniProt accession no.: P00387
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... CYB5R3(1727)

Description

General description

NADH-cytochrome b5 reductase 3 (EC 1.6.2.2; UniProt P00387; also known as B5R, Cytochrome b5 reductase, Diaphorase-1) is encoded by the CYB5R3 (also known as DIA1) gene (Gene ID 1727) in human. Cytochrome b5 reductase is a flavoprotein that serves as electron donor for cytochrome b5, a ubiquitous electron carrier and participates in a variety of metabolic pathways, including desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. It is produced as two different isoforms that have different localizations. The membrane-bound form is found in somatic cells where it is anchored to the endoplasmic reticulum and mitochondrial membranes. The soluble form is found in erythrocytes. Mutations in the CYB5R3 gene result in deficiency in NADH-cytochrome b5 reductase+C20 in erythrocytes, which causes methemoglobinemia types I and II that are characterized by the presence of excessive amounts of methemoglobin in blood cells and their reduced capacity to carry oxygen. Ref.: Elahian, F., et al. (2014). Crit. Rev Biotechnol. 34, 134-143.

Specificity

Target epitope is present in both the ER/mitochondria membrane-bound and cytosolic forms of human CYB5R3.

Immunogen

GST-tagged recombinant human CYB5R3 C-terminal fragment.

Application

Detect CYB5R3 using this mouse monoclonal Anti-CYB5R3 Antibody, clone 1D9.2, Cat. No. MABS1727, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected CYB5R3 in human kidney, skeletal muscle, and tonsil tissue sections.

Quality

Evaluated by Western Blotting in HepG2 celll lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CYB5R3 in 10 µg of HepG2 cell lysate.

Target description

~34 kDa observed. 34.10 kDa (isoform 1 or M; Membrane-bound form with Met1 removed, a.a. 2-301), 31.26 kDa (Cytosolic form, a.a. 27-301), 31.63 kDa (isoform 2 or S), 38.23 kDa (isoform 3) calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1