Acetyl-coenzyme A synthetase, cytoplasmic (EC 6.2.1.1; UniProt Q9NR19; also known as Acetate-CoA ligase, Acetyl-CoA synthetase, AceCS, ACS, Acyl-activating enzyme, Acyl-CoA synthetase short-chain family member 2) is encoded by the ACSS2 (also known as ACAS2) gene (Gene ID 55902) in human. ACS plays a vital role in prokaryotic and eukaryotic cells by activating acetate to form acetyl CoA that serves as a source of two carbon metabolite for lipid synthesis and energy producing reactions. It is also reported to play a role in gene expression. In yeast cells it delivers acetyl CoA for histone acetylation. ACS contributes to cancer cell growth under low-oxygen and lipid-depleted conditions and its expression is upregulated under metabolically stress. Silencing ACSS2 gene is shown to reduce the growth of tumor xenografts. Ref.: Starai, VJ., and Escalante-Semerena, JC. (2004). Cell. Mol. Life Sci. 61, 2020 2030. Schug, ZT et al. (2015). Cancer Cell. 27:57-71.
Specificity
Immunogen sequence is present in both spliced isoforms of human acetyl-CoA synthetase reported by UniProt (Q9NR19).
Immunogen
GST-tagged recombinant human acetyl-CoA synthetase N-terminal fragment.
Application
Detect Acetyl-CoA synthetase using this mouse monoclonal Anti-Acetyl-CoA Synthetase Antibody, clone 6C1.2, Cat. No. MABS1720, validated for use in Western Blotting.
Quality
Evaluated by Western Blotting in HEPG2 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected acetyl-CoA synthetase in 10 µg of HepG2 cell lysate.
Target description
~80 kDa observed. 78.58/80.09 kDa (isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Other Notes
Concentration: Please refer to lot specific datasheet.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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