MABS1719

Anti-TMEM64 Antibody, clone 7A2.1

clone 7A2.1, from mouse

• Synonym(s): Transmembrane protein 64
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 7A2.1, monoclonal
• species reactivity: human
• species reactivity (predicted by homology): rat (based on 100% sequence homology)
• technique(s): western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_001008495
• UniProt accession no.: Q6YI46
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... TMEM64(169200)

Description

General description

Transmembrane protein 64 (UniProt Q6YI46) is encoded by the TMEM64 gene (Gene ID 169200) in human. TMEM64 is a 6-transmembrane protein that plays a role in modulating Wnt/ -catenin signaling-mediated osteoblast and adipocyte differentiation. TMEM64 interacts and positively regulates sarcoplasmic endoplasmic reticulum Ca(2+) ATPase 2 (SERCA2) activity, which is important for RANKL-induced Ca(2+) oscillation, Ca(2+)/calmodulin-dependent protein kinases (CaMK) IV activity and mitochondrial ROS formation needed for for osteoclast formation. Mice with Tmem64 deficiency are characterized by a high-bone-mass phenotype, displaying higher levels of ALP, osteocalcin (Ocn), and type 1 collagen (Col 1a) transcripts than in wild-type mice. Tmem64-knockout in mice is reported to markedly enhance osteoblast differentiation and inhibit adipocyte differentiation from bone marrow-derived stromal cells (BMSCs), resulting in a preferential switch of mesenchymal progenitors favoring the osteogenic over the adipogenic lineage.

Specificity

The immunogen sequence is 100% conserved between human and rat species and present in all four human spliced isoforms reported by UniProt (Q6YI46 & D3ZV19). Murine cross-reactivity is highly likely, but has not been confirmed.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminal end sequence of human and rat TMEM64.

Application

Detect TMEM64 using this mouse monoclonal Anti-TMEM64, clone 7A2.1 Antibody, Cat. No. MABS1719, validated for use in Western Blotting.

Research Category
Signaling

Western Blotting Analysis: 2 µg/mL of this antibody detected TMEM64 in 10 µg of SK-MEL-28 cell lysate.

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected TMEM64 in 10 µg of A431 cell lysate.

Target description

~45 kDa observed. 39.67/12.95/14.25/33.97 kDa (human isoform 1/2/3/4) and 39.53 kDa (rat) calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified.

Purified mouse IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable