MABS1717
Anti-FNIP1 Antibody, clone 1C10.2
clone 1C10.2, from mouse
Synonym(s):
Folliculin-interacting protein 1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1C10.2, monoclonal
species reactivity
human
technique(s)
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... FNIP1(96459)
General description
Folliculin-interacting protein 1 (UniProt Q8TF40; FNIP1 protein) is encoded by the FNIP1 (also known as KIAA1961) gene (Gene ID 96459) in human. FNIP1 and 2 proteins are homologous partners of folliculin (FLCN) and are required for FLCN localization to lysosomes during amino acid starvation. FNIP1 protein is an evolutionary conserved 130 kDa protein that is shown to interact with AMPK and can serve as a substrate for activated AMPK. Blocking of AMPK activity is reported to reduce FNIP protein expression in HEK293 cells. FNIP1 is also involved in autophagic process where it interacts with GABARAP of the ATG8 family and knockdown of FNIP1 is shown to enhance autophagosome formation. Strong expression of FNIP1 protein is reported in the heart, liver placenta, muscle, nasal mucosa, salivary gland and uvula and moderate expression in kidney and lung. Higher levels have been reported in renal cell carcinoma (RCC). Loss of FNIP1 protein is also shown to mitigate muscle damage in a murine model of Duchenne muscular dystrophy. Ref.: Baba, M., et al. (2006). Proc. Natl. Acad. Sci. USA 103, 15552 15557. Hasumi, H., et al. (2015). Proc. Natl. Acad. Sci. USA 112, 1624-1631.
Specificity
Epitope region is present in spliced isoforms 1 and 3, but not 2, of human FNIP1 reported by UniProt (Q8TF40).
Immunogen
GST-tagged recombinant fragment corresponding to a region within the C-terminal half of human FNIP1.
Application
Detect FNIP1 using this mouse monoclonal Anti-FNIP1 Antibody, clone 1C10.2, Cat. No. MABS1717, validated for use in Western Blotting.
Research Category
Signaling
Signaling
Quality
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FNIP1 in 10 µg of HEK293 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FNIP1 in 10 µg of HEK293 cell lysate.
Target description
~130 kDa observed. 130.6/127.4 kDa (isoform 1/3) calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Protein G purified.
Purified mouse IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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J-M Heo et al.
Science advances, 4(11), eaav0443-eaav0443 (2019-01-11)
Removal of damaged mitochondria is orchestrated by a pathway involving the PINK1 kinase and the PARKIN ubiquitin ligase. Ubiquitin chains assembled by PARKIN on the mitochondrial outer membrane recruit autophagy cargo receptors in complexes with TBK1 protein kinase. While TBK1
Yunlong Zhang et al.
FEBS letters, 595(1), 123-132 (2020-10-17)
Folliculin (FLCN) is a tumor suppressor protein involved in many cellular processes, including cell signaling, apoptosis, and autophagy. In ciliated cells, FLCN localizes to primary cilia and controls mTORC1 signaling in response to flow stress. Here, we show that the
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