MABS1290

Anti-LANCL2 Antibody, clone 392

clone 392, from mouse

• Synonym(s): Testis-specific adriamycin sensitivity protein
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 392, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_061167
• UniProt accession no.: Q9NS86
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... LANCL2(55915)

Description

General description

LanC-like protein 2 (UniProt: Q9NS86; also known as Testis-specific adriamycin sensitivity protein) is encoded by the LANCL2 (also known as GPR69B, TASP) gene (Gene ID: 55915) in human. LANC2 is a member of the highly conserved and widely distributed lanthionine synthetase component C-like (LANCL) protein family. It is well expressed in brain and testis and is localized to the plasma membrane, juxta-nuclear vesicles, and the nucleus. The N-terminus of LANCL2 differs from the other LANCL proteins by an N-terminal extension of 17 amino acid residues and an insertion of 19 residues. LANCL2 contains 15 cysteine residues, three of which are conserved in all human LANCL proteins and 8 are conserved between LANCL2 and LANCL1. LANCL2 displays about 75% amino acid homology with LANCL1 protein. LANCL2 contains a phosphatidylinositol phosphate (PIP) binding site at the N-terminus and its myristoylation is essential for its membrane association. LANCL2 is essential for abscisic acid (ABA) binding on the cell membrane and activation of the ABA signaling pathway in granulocytes. Exogenous expression LANCL2 in a sarcoma cell line is shown to reduce the expression of ABCB1 (P-glycoprotein 1) and increase cellular sensitivity to adriamycin. (Ref.: Sturla, L et al. (2009). J. Biol. Chem. 284(41):28045-57; Ladlinger, C et al. (2006). Biochim Biophys Acta. 1758(11):1759-67).

Specificity

Clone 392 detected LANCL2 overexpressed in HEK293 cells.

Immunogen

Epitope: unknown

GST-tagged full length human recombinant LANCL2 protein.

Application

Detect LANCL2 using this mouse monoclonal Anti-LANCL2 Antibody, clone 392, Cat. No. MABS1290. It is used in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A representative lot detected LANCL2 in Immunocytochemistry applications (Fresia, C., et. al. (2016). Sci Rep. 6:26658).

Western Blotting Analysis: A representative lot detected LANCL2 in Western Blotting applications (Vigliarolo, T., et. al. (2015). J Biol Chem. 290(21):13042-52; Vigliarolo, T., et. al. (2016). Int J Biochem Cell Biol. 75:99-103).

Research Category
Signaling

Quality

Evaluated by Western Blotting in lysates from HEK293 cells overexpressing LANCL2.

Western Blotting Analysis: 4 µg/mL of this antibody detected LANCL2 in 10 µg of cell lysate from HEK293 cells overexpressing LANCL2.

Target description

~50 kDa observed; 50.85 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein A purified

Purified mouse monoclonal antibody IgG1 in PBS with 0.02% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable