MABS1241

Anti-Synembryn-A/Ric8A Antibody, clone 3E1

clone 3E1, from mouse

• Synonym(s): Protein Ric-8A, Resistance to inhibitors of cholinesterase 8
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 3E1, monoclonal
• species reactivity: rat, human
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG2aκ
• UniProt accession no.: Q80ZG1
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... RIC8A(60626)

Description

General description

Synembryn-A (UniProt: Q80ZG1; also known as Resistance to inhibitors of cholinesterase 8, Protein Ric-8A) is encoded by the Ric8a gene (Gene ID: 293614) in rat species. Resistance to inhibitors of cholinesterase 8 (RIC-8) is conserved in vertebrates and is concentrated in the cytoplasm of neurons. Mammalian Ric-8 proteins are G alpha subunit guanine nucleotide exchange factors. Ric-8A and Ric-8B collectively stimulate nucleotide exchange of all G alpha subunit classes by stabilizing the G alpha nucleotide-free transition state. Ric-8 is reported to directly participate in the post-translational folding of nascent G alpha subunits to promote G-protein biosynthesis. It may facilitate the transition of G alpha from a pre-folded globular state to its native state by promoting the first G alpha guanine nucleotide-binding event. Ric-8 interacts with GDP-bound G alpha proteins, but does not interact with G-alpha proteins when they are in complex with beta and gamma subunits. Overexpression of Ric-8 proteins in HEK293, NIH 3T3, or Sf9 cells is shown to enhance cellular G alpha levels. Embryonic stem cells lacking Ric-8A or Ric-8B produce defective G alpha subunits that turn over at about 10-fold higher rates than in wild-type cells. Ref.: Thomas, C.J. et al. (2011). PLoS ONE 6(8): e23197.

Specificity

Clone 3E1 detects Ric-8A in human brain.

Immunogen

A full-length recombinant Ric-8A protein from rat.

Application

Detect Synembryn-A/Ric-8A using this mouse monoclonal Anti-Synembryn-A/Ric8A antibody, clone 3E1, Cat. No. MABS1241, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Synembryn-A/Ric-8A in human cerebral cortex, human cerebellum, and human testis tissues.

Western Blotting Analysis: A representative lot detected recombinant Synembryn-A/Ric-8A protein (Chan, P.Y., et al. (2013). Proc. Natl. Acad. Sci. USA. 110(10):3794-3799).

Quality

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Synembryn-A/Ric-8A in 10 µg of human brain tissue lysate.

Target description

~60 kDa observed; 59.7 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1