MABS1189

Anti-PP2C Isoform delta Antibody, clone 2F7.1

clone 2F7.1, from mouse

• Synonym(s): Protein phosphatase 1D, O15297, Wild-type p53-induced protein phosphatase 1, PP2C-delta, Protein phosphatase 2C isoform delta, Protein phosphatase magnesium-dependent 1 delta
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 2F7.1, monoclonal
• species reactivity: human, rat
• species reactivity (predicted by homology): mouse (based on 100% sequence homology)
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_003611
• UniProt accession no.: O15297
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... PPM1D(8493)

Description

General description

Protein phosphatase 1D (EC 3.1.3.16; UniProt O15297; also known as Wild-type p53-induced protein phosphatase 1, PP2C-delta, Protein phosphatase 2C isoform delta, Protein phosphatase magnesium-dependent 1 delta) is encoded by the PPM1D (also known as WIP1) gene (Gene ID 8493) in human. Wild-type p53-induced phosphatase 1 (WIP1) or PP2C-delta is a serine/threonine phosphatase. Unlike PP2A or PP1 enzymes, WIP1 functions as a monomer whose catalytic activity is dependents on Mg2+ or Mn2+ and is insensitive to inhibition by okadaic acid or microcystin. Casein kinase 2 (CK2) andPP2C-delta catalyze the phosphorylation and dephosphorylation, respectively, of LSD1 on Ser131 and Ser137 residues. S131/S137 phosphorylation promots LSD1 interaction with RNF168 and RNF168-dependent 53BP1 ubiquitination and subsequent recruitment to DNA damage sites. Other known PP2C-delta targets include ATM (S367, S1981), -H2AX, CHK1 (S345) and CHK2 (T68).

Specificity

Clone 2F7.1 targets an internal epitope conserved among the two human spliced isoforms and mouse/rat PP2C-delta (Uniprot O15297, B1WCA0, Q9QZ67).

Immunogen

GST-tagged recombinant human PP2C-delta internal fragment.

Application

Anti-PP2C Isoform delta Antibody, clone 2F7.1, Cat. No. MABS1189, is a highly specific mouse monoclonal antibody, that targets PPM1D and has been tested in Western Blotting and Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected PP2C-delta in human prostate cancer, normal human brain and rat testis tissue sections.

Research Category
Epigenetics & Nuclear Function

Quality

Evaluated by Western Blotting in HL-60 human promyelocytic leukemia cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected PP2C-delta in 10 µg of HL-60 human promyelocytic leukemia cell lysate.

Target description

~60 kDa observed. 66.68/47.21 kDa (human isoform 1/2), 65.72 kDa (mouse), and 65.68 kDa (rat) calculated.

Physical form

Format: Purified

Protein G purified.

Purified mouse monoclonal antibody IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable