MABS1161

Anti-PYCR1 Antibody, clone 2D9.1

clone 2D9.1, from mouse

• Synonym(s): Pyrroline-5-carboxylate reductase 1, mitochondrial, PYCR1, P5C reductase 1, P5CR 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2D9.1, monoclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_001269209
• UniProt accession no.: P32322
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PYCR1(5831)

Description

General description

Pyrroline-5-carboxylate reductase 1, mitochondrial (EC 1.5.1.2; UniProt P32322; also known as P5C reductase 1, P5CR 1) is encoded by the PYCR1 (also known as ARCL2B, ARCL3B) gene (Gene ID 5831) in human. Pyrroline-5-carboxylate (P5C) reductase (PYCR1) is a mitochondrial enzyme that utilizes NAD(P)H as cofactor and catalyzes the convertion of P5C to L-proline, as well as the coversion of Δ1-piperideine-6-carboxylate (P6C) to L-pipecolic acid, both of which are intermediates in the catabolism pathway of the essential amino acid L-lysine. PYCR1 gene mutations are associated with the autosomal recessive disorder cutis laxa type II, menifested with premature aging features such as joint laxity and wrinkled/lax skin, as well as developmental delay.

Specificity

Expected to react with spliced isoforms 1 and 3, but not isoform 2, reported by UniProt P32322).

Immunogen

GST-tagged recombinant protein corresponding to the C-terminal of human PYCR1.

Application

Anti-PYCR1 Antibody, clone 2D9.1 is an antibody against PYCR1 for use in Western Blotting, Immunohistochemistry (Paraffin).

Quality

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PYCR1 in 10 µg of K562 cell lysate.

Target description

~33 kDa observed. 33.36 kDa (isoform 1) and 35.98 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable