MABS1137

Anti-Phosphotyrosine, clone RM111 Antibody

clone RM111, from rabbit, purified by affinity chromatography

• Synonym(s): p-Tyr, ptyr, phospho-tyrosine
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: RM111, monoclonal
• purified by: affinity chromatography
• species reactivity: monkey
• species reactivity (predicted by homology): all
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG
• shipped in: wet ice
• target post-translational modification: phosphorylation (pTyr)

Description

General description

The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

Immunogen

recombinant protein corresponding to All Phosphotyrosine Protein.

Application

Anti-Phosphotyrosine, clone RM111 is an antibody against Phosphotyrosine for use in WB, ICC, IP, IH.

Immunoprecipitation Analysis: 1:1000 dilution of this antibody immunoprecipitated phosphotyrosine modified proteins from EGF-treated A431 cell lysate
Immunocytochemistry Analysis: 1:500 dilution of this antibody detected phosphotyrosine modified proteins from serum-starved A431 cells treated with EGF
Immunohistochemistry Analysis: 1:500 dilution of this antibody detected phosphotyrosine modified proteins from human lung cancer tissue
Luminex Analysis: This antibody demonstrated to only react with phosphorylated tyrosine when tested against a phospho and non-phospho amino acid panel

Research Category
Signaling

Research Sub Category
General Post-translation Modification

Quality

Evaluated by Western Blotting on EGF treated Cos7 cell lysate
Western Blotting Analysis: 1:1000 of this antibody detected phosphotyrosine modified proteins from 10 µg of EGF treated Cos7 cell lysate

Target description

N/A kDa observed

Physical form

Protein A purified

Purified rabbit monoclonal IgG in PBS buffer containing 1% BSA and 0.09% sodium azide

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable