MABN661

Anti-QKI-5 Antibody, clone N195A/16

clone N195A/16, from mouse

• Synonym(s): Protein quaking, Hqk, HqkI
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: N195A/16, monoclonal
• species reactivity: rat, human
• species reactivity (predicted by homology): mouse (based on 100% sequence homology)
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_006766
• UniProt accession no.: Q96PU8
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... QKI(9444)

Description

General description

QKI (Quaking) is an RNA-binding protein that plays a central role in myelinization. QKI acts by regulating pre-mRNA splicing, mRNA export, mRNA stability, and protein translation. It is regulated by alternative splicing. There are at least 6 known isoforms, and QKI-5 refers to isoforms 1, which is the ′canonical′ sequence, consisting of 341 amino acids.

Specificity

This antibody does not cross react with QKI-6, QKI-7, and QKI-7b. This antibody may cross react with other STAR proteins containing similar nuclear localization signals (Prof. J. Trimmer, Univerisity of California, Davis.).

Immunogen

Linear peptide corresponding to human QKI-5.

Application

Detect QKI using this mouse monoclonal antibody, Anti-QKI-5 Antibody, clone N195A/16 validated for use in IHC & western blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected QKI-5 in human cerebellum and thalamus tissues, and in rat hippocampus tissue.

Western Blotting Analysis: A representative lot detected QKI-5 in COS cell lysates transiently transfected with Flag-tagged QKI-5 (Prof. J. Trimmer, University of California, Davis.).

Quality

Evaluated by Immunohistochemistry in rat cerebellum tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected QKI-5 in rat cerebellum tissue.

Target description

45 kDa calculated

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable