MABN650

Anti-Kirrel3 Antibody, short isoform 3, clone N320/48

clone N320/48, from mouse

• Synonym(s): Kin of IRRE-like protein 3, Kin of irregular chiasm-like protein 3, Processed kin of IRRE-like protein 3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: N320/48, monoclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001177840
• UniProt accession no.: Q8BR86-3
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... KIRREL3(84623)

Description

General description

Kin of IRRE-like protein 3 (Kirrel3) or Kin of irregular chiasm-like protein 3 is expressed primarily in adult brain, bone marrow and stromal cells. Kirrel3 is thought to be involved in the hematopoietic supportive capacity of stroma cells, and the secreted extracellular domain of Kirrel3 is directly responsible for supporting hematopoietic stem cells.

Specificity

This antibody recognizes the cytoplasmic domain of Kirrel3, short isoform 3 and does not cross-react with other Kirrel3 isoforms.

Immunogen

Linear peptide corresponding to the cytoplasmic domain of mouse Kirrel3, short isoform 3.

Application

Detect Kin of IRRE-like protein 3 using this mouse monoclonal antibody, Anti-Kirrel3 Antibody, short isoform 3, clone N320/48 validated for use in western blotting & IHC.

Western Blotting Analysis: A representative lot detected Kirrel3, short isoform 3 in COS cell lysates transiently transfected with Kirrel3, short form, and demonstrated a loss of signal in COS cell lyasates transiently transfected with Kirrel3, long form or with untagged Kv2.1 plasmids (Prof. J. Trimmer, University of California, Davis.).
Immunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected Kirrel3, short isoform 3 in human thalamus and human bone marrow tissue.

Quality

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Kirrel3, short isoform 3 in 10 µg of human brain tissue lysate.

Target description

~ 75 kDa observed

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class: 10 - Combustible liquids