MABN615

Anti-Pan-GRK Antibody, clone N145/20

clone N145/20, from mouse

• Synonym(s): G protein-coupled receptor kinase 5, G protein-coupled receptor kinase GRK5
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: N145/20, monoclonal
• species reactivity: mouse
• technique(s): western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_061357
• UniProt accession no.: Q8VEB1
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Grk5(14773)

Description

General description

G protein-coupled receptor kinases (GRK) are serine/threonine kinases that phosphorylate a variety of G-protein-coupled receptors (GPCRs), specifically activated forms, including adrenergic receptors, muscarinic acetylcholine receptors, dopamine receptors and opioid receptors. GRK receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation.

Specificity

This antibody recognizes the C-terminus of GRK. This antibody cross reacts with GRK6 (Prof. J. Trimmer, University of California, Davis.).

Immunogen

Epitope: C-terminus

Recombinant protein corresponding to the C-terminus of mouse Pan-GRK.

Application

Anti-Pan-GRK Antibody, clone N145/20 Cat. No. MABN615 is a highly specific mouse monoclonal antibody & that targets GRK & has been tested in western blotting.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blotting in mouse liver tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Pan-GRK in 10 µg of mouse liver tissue lysate.

Target description

~ 68 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse liver tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable