MABN613

Anti-Potassium channel Kv2.1 Antibody, clone L80/21

clone L80/21, from mouse

• Synonym(s): Potassium voltage-gated channel subfamily B member 1, Delayed rectifier potassium channel 1, DRK1, Voltage-gated potassium channel subunit Kv2.1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: L80/21, monoclonal
• species reactivity: rat, mouse
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG3κ
• NCBI accession no.: NP_037318
• UniProt accession no.: P15387
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Kcnb1(16500)

Description

General description

Voltage-gated potassium channel subunit Kv2.1 (Kv2.1) is also called potassium voltage-gated channel subfamily B member 1 (KCNB1) or delayed rectifier potassium channel 1 (DRK1). Kv2.1 is a voltage-gated potassium ion channel that is expressed in the brain (cell bodies and dendrites of neurons but not axons, as well as Schwann cells), atria, ventricle, skeletal muscle, cochlea, retina, lung and pulmonary arteries. It is involved in maintaining membrane potential and modulating electrical excitability of both neurons and muscle cells. It is thought that Kv2.1 has a role in the muscle tone of the pulmonary system, as the expression Kv2.1 in pulmonary smooth muscle has been found to be reduced in chronic hypoxic pulmonary hypertension.

Specificity

This antibody recognizes the C-terminus of Potassium channel Kv2.1.

Immunogen

Epitope: C-terminus

Linear peptide corresponding to the C-terminus of rat Potassium channel Kv2.1.

Application

Detect Kv2.1 using this mouse monoclonal antibody, Anti-Potassium channel Kv2.1 Antibody, clone L80/21 validated for use in western blotting & IHC (Paraffin).

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Potassium channel Kv2.1 in rat frontal cortex tissue.

Western Blotting Analysis: A representative lot detected Potassium channel Kv2.1 in wild type mouse brain membrane tissue lysate, and demonstrated a loss of signal in Potassium channel Kv2.1 knock out mouse brain membrante tissue lysate (Prof. J. Trimmer, University of California, Davis.).

Research Category
Neuroscience

Research Sub Category
Synapse & Synaptic Biology

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Potassium channel Kv2.1 in 10 µg of rat brain tissue lysate.

Target description

~95 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable