MABN528

Anti-Glypican-4 Antibody, clone 1C7.2

culture supernatant, clone 1C7.2, from mouse

• Synonym(s): Glypican-4, K-glypican, Secreted glypican-4
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: 1C7.2, monoclonal
• species reactivity: human, mouse
• technique(s): western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_001439
• UniProt accession no.: O75487
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... GPC4(2239)

Description

General description

Glypican-4 is a glycosylphosphatidylinositol (GPI)-anchored cell surface heparan sulfate proteoglycan (HSPG) that is encoded by the GPC4 gene in human. Glypican-4 belongs to the family of glypican-related integral membrane proteoglycans (GRIPs) that may be involved in the control of cell division and growth regulation. Deletion of the entire GPC4 gene and the two exons at the 3′ end of the adjacent GPC3 gene has been reported in a patient diagnosed with Simpson-Golabi-Behmel syndrome (SGBS).

Immunogen

GST-tagged recombinant protein corresponding to human Glypican-4.

Application

Anti-Glypican-4 Antibody, clone 1C7.2 is an antibody against Glypican-4 for use in Western Blotting.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Western Blotting Analysis: A 1:500 dilution from a representative lot detected Glypican-4 in 10 µg of mouse whole embryo, mouse E16 brain, and human kidney tissue lysate.

Quality

Evaluated by Western Blotting in mouse E17 spinal cord tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Glypican-4 in 10 µg of mouse E17 spinal cord tissue lysate.

Target description

~62 kDa observed

Physical form

Mouse monoclonal IgG2bκ in tissue culture supernatant containing 0.05% sodium azide

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10-13 - German Storage Class 10 to 13
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable