MABN462

Anti-ASIC1 Antibody, clone N271/44

clone N271/44, from mouse

• Synonym(s): Acid-sensing ion channel 1, ASIC1, Acid-sensing ion channel, Amiloride-sensitive cation channel 2, neuronal, Brain sodium channel 2, BNaC2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: N271/44, monoclonal
• species reactivity: rat, mouse
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_033727
• UniProt accession no.: Q6NXK8
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: rat ... Asic1(79123)

Description

General description

Acid-sensing ion channels (ASICs) are broadly distributed in the nervous system where they contribute to certain sensory processes. In particular, they survey moderate decreases in extracellular pH as a means to regulate acid levels and acid-induced pain. ASICs thereby serve as a popular target for the treatment of acidosis. The acid-sensing ion channel 1 consists of a trimeric protein, where each subunit is composed of two transmembrane spanning helices, a highly organized ectodomain, and an intracellular N-terminus and C-terminus.

Specificity

This antibody recognizes the C-terminus of ASIC1.

Immunogen

Epitope: C-terminus

Recombinant protein corresponding to the C-terminus of mouse ASIC1.

Application

Anti-ASIC1 Antibody, clone N271/44 detects level of ASIC1 & has been published & validated for use in Western Blotting & IHC.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Western Blot: A representative lot detected ASIC1 in WT mice, but demonstrated a loss of signal in ASIC1 knockout mice.

Immunohistochemistry Analysis: A representative lot detected ASIC1 in rat brain tissue (Prof. J. Trimmer, University of California, Davis).

Quality

Evaluated by Western Blot in rat brain tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected ASIC1 in 10 µg of rat brain tissue lysate.

Target description

~60 kDa observed. Two isoforms at ~60 kDa and ~62 kDa are known to exist.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable