MABN461

Anti-Dicer1 Antibody, clone N167/7

clone N167/7, from mouse

• Synonym(s): Endoribonuclease Dicer, Double-strand-specific ribonuclease mDCR-1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: N167/7, monoclonal
• species reactivity: rat, human, mouse
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_683750
• UniProt accession no.: Q8R418
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Dicer1(192119)

Description

General description

RNA interference (RNAi) is mechanism for post-transcriptional gene silencing that leads to the loss of the activity of the gene. RNAi is activated when long dsRNAs are cleaved by the enzyme Dicer1 (RNase III family of dsRNA-specific endonucleases) into 21-23 nucleotide siRNA (small interfering RNA). These siRNAs bind proteins to form the RISC (RNA-induced silencing complex) which is activated when the double stranded siRNAs are unzipped to single stranded siRNAs. Activated RISC recognizes and binds complementary sequences in mRNA. The mRNA is cleaved and the overall effect is an inhibition of the protein expression and a loss of the gene activity. Dicer1 along with the Argonaute family of proteins (Ago1 and Ago2) share the same domain PIWI and PAZ domain that provide endonuclease activity to RNA-induced silencing complexes (RISC).

Immunogen

Recombinant protein corresponding to mouse Dicer1.

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Dicer1 in human ovarian adenocarcinoma tissues and in rat cerebellum tissue.

Western Blot Analysis: A representative lot from an independent laboratory detected Dicer1 in adult rat brain membrane and in WT mouse brain lysate, and demonstrated a loss of signal in Dicer1 knockout mouse brain lysate.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

This Dicer1 antibody is validated for use in WB & IHC for the detection of the Dicer1 protein.

Quality

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Dicer1 in 10 µg of MCF-7 cell lysate.

Target description

~217 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
MCF-7 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable