Adenosine deaminase, also known as ADA, or Cat eye syndrome critical region protein 1, and encoded by the gene CECR1/ADA2/ADGF/IDGFL, is an extracellular enzyme that works on adenosine and causes the degradation of adenosine to inosine and free NH3. Adenosine deaminase is an extracellular secreted enzyme that binds to cell surfaces via proteoglycans. The binding appears to mediate cellular proliferation and differentiation in many systems, though this activity is independent of its deaminase activity. Adenosine deaminase is highly present in blood plasma and widely expressed in adult and fetal tissues. While there many clinical deficiencies associated with adenosine deaminase (ADA) mutations, the level of ADA activity in the blood and fluids is used as a biomarker to help distinguish various diseases. For instance, the level of activity in lung fluid is correlated with tuberculosis infection and used to distinguish it from malignant pleural effusion (MPE). The ADA level in CSF in patients with meningitis also helps to identify tuberculosis as the cause as opposed to another agent or condition.
Immunogen
GST-tagged recombinant protein corresponding to human Adenosine Deaminase.
Application
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Adenosine Deaminase in human placenta tissue.
Research Category Neuroscience
Research Sub Category Developmental Signaling
This Anti-Adenosine Deaminase Antibody, clone 9H12.1 is validated for use in western blotting & IHC (Paraffin) for the detection of Adenosine Deaminase.
Quality
Evaluated by Western Blotting in human placenta tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Adenosine Deaminase in 10 µg of human placenta tissue lysate.
Target description
~ 60 kDa observed
Physical form
Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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