Ectoderm-neural cortex protein 1 (ENC1) as its name suggests is a protein involved in the regulation and differentiation of neural crest cells and neuronal development, as well as a protein involved in neuronal process formation later on. It is also known as nuclear matrix protein NRP/B. It is an actin binding protein that appears important in the organization of the actin cytoskeleton during neural fate specification and nervous system development. Its role in neuronal process formation is via its binding of RB1 (p110RB) when it is hypophosophorylated. ENC1 is widely expressed during development in the fetal heart, lung, and kidney. In the adult ENC1 is more restricted to nervous tissues primarily. It is abundant in adult brain, hippocampus, amygdale and spinal cord. There is also expression found in the adult pancreas. Numerous neuronal cancer cell lines and primary brain tumors also express ENC1. Localization is nuclear for the most part but in some cancer cells cytoplasmic staining is also observed. Its role in tumors is as an oncogene as studies suggest that both amplification and alteration of ENC1 may lead to tumor development and growth. Moreover ENC1 plays a role in the oxidative stress response as a regulator of the transcription factor Nrf2, and expression of this gene may play a role in malignant transformation as well.
Immunogen
GST-tagged recombinant protein corresponding to human ENC-1.
Application
Anti-ENC-1 Antibody, clone 1H8.1 is a highly specific mouse monoclonal antibody, that targets Ectoderm-neural cortex-1 & has been tested in western blotting & IHC.
Western Blotting Analysis: 1 µg/mL from a representative lot detected ENC-1 in 10 µg of rat hippocampus tissue.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ENC-1 in human cerebellum and cerebral cortex tissue.
Quality
Evaluated by Western Blotting in human hippocampus tissue.
Western Blotting Analysis: 1 µg/mL of this antibody detected ENC-1 in 10 µg of human hippocampus tissue.
Target description
~66 kDa observed
Linkage
Replaces: MABC685
Physical form
Format: Purified
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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