MABN376

Anti-Alanyl-tRNA synthetase (AARS Antibody), clone M6-P2E5

clone P2E5, 1 mg/mL, from mouse

• Synonym(s): Alanine-tRNA ligase, cytoplasmic, Alanyl-tRNA synthetase, AlaRS, Renal carcinoma antigen NY-REN-42
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: P2E5, monoclonal
• species reactivity: mouse, human
• concentration: 1 mg/mL
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001596
• UniProt accession no.: P49588
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... AARS1(16)

Description

General description

Alanyl-tRNA synthetase (AARS), also known as Alanine-tRNA ligase, AlaRS, or Renal carcinoma antigen NY-REN-42, and encoded by the gene AARS, is a key transfer RNA synthetase responsible for the attachment of alanine to the tRNA(Ala) for protein translation. Alanine-tRNA ligase also can edit incorrectly charged tRNA(Ala) for proper translation. Alanine-tRNA ligase is a monomer and is localized in the cytoplasm and ubiquitously expressed. Mutations in Alanine-tRNA ligase have been linked to a progressive peripheral nervous disorder called Charcot-Marie Tooth disease. Moreover autoantibodies against aminoacyl tRNA synthetases are some of the most common autoantibodies detected in autoimmune diseases with nearly 25-35% of patients having them, and some of these such as autoantibodies to Alanine-tRNA ligase have also been associated with autologous antibodies in renal carcinoma patients.

Immunogen

Linear peptide corresponding to the C-terminus of human Alanyl-tRNA synthetase (AARS).

Application

This Anti-Alanyl-tRNA synthetase (AARS Antibody), clone M6-P2E5 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Alanyl-tRNA synthetase (AARS).

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Alanyl-tRNA synthetase (AARS) in 10 µg of HepG2 cell lysate.
Immunohistochemistry Analysis: A representative lot detected Alanyl-tRNA synthetase (AARS) in human breast cancer tissue (Prof. Ayham Alnabulsi, University of Aberdeen)

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Alanyl-tRNA synthetase (AARS) in 10 µg of HeLa cell lysate.

Target description

~110 kDa observed

Physical form

Format: Purified

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable