MABN1725

Anti-Thyroid Hormone Receptor β-1 Antibody, clone N402/46

clone N402/46, from mouse

• Synonym(s): Thyroid hormone receptor beta, Nuclear receptor subfamily 1 group A member 2, c-erbA-2, c-erbA-beta
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: N402/46, monoclonal
• species reactivity: mouse, rat, human
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_000452
• UniProt accession no.: P10828
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... THRB(7068)

Description

General description

Thyroid hormone receptor beta (UniProt: P10828; also known as Nuclear receptor subfamily 1 group A member 2, c-erbA-2, c-erbA-beta) is encoded by the THRB (also known as ERBA2, NR1A2, THR1) gene (Gene ID: 7068) in human. Thyroid hormone receptors (TRs) are ligand dependent members of the steroid/retinoic acid superfamily of transcription factors. Thyroid hormone receptor beta is a nuclear hormone receptor that has a transactivation domain at its N-terminal, a ligand-binding domain at the C-terminal, and a DNA-binding domain that binds to sequences of promoter DNA known as hormone response elements. It serves as a high affinity receptor for thyroid hormones, including triiodothyronine (T3) and thyroxine and serves as a hormone-dependent transcriptional regulator that binds DNA sequences and is activated by T3. It plays an important role in vertebrate development, specifically in developing limbs and face. 9-cis-retinoic acid receptor (RXR) and retinoic acid receptor (RAR) receptor families are often associated with thyroid hormone receptor -1 and form homodimers and heterodimers. Mutations in this gene are known to be a cause of generalized thyroid hormone resistance (GTHR), a syndrome characterized by goiter and high levels of circulating thyroid hormone (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH).

Specificity

Clone N402/46 detects Thyroid Hormone Receptor beta-1 in multiple human tissues.

Immunogen

A full-length human recombinant Thyroid Hormone Receptor beta-1.

Application

Anti-Thyroid Hormone Receptor -1, clone N402/46 Antibody, Cat. No. MABN1725, is a highly specific mouse monoclonal antibody that targets Thyroid Hormone Receptor -1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Thyroid Hormone Receptor -1 in human pancreas and human liver tissue.

Western Blotting Analysis: A representative lot detected Thyroid Hormone Receptor beta-1 in COS cells transiently transfected with Myc-tagged TRalpha1, Myc-tagged TRbeta1, Myc-tagged TRbeta2 or untagged Kv2.1 plasmid. (Courtesy of James Trimmer, Ph.D., University of California at Davis, USA).

Research Category
Neuroscience

Quality

Evaluated by Immunohistochemistry in human cerebellum tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Thyroid Hormone Receptor -1 in human cerebellum tissue.

Target description

~60 kDa observed; 52.79 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable