MABN1540

Anti-P2RX7 Antibody, clone 14H7.1

clone 14H7.1, from mouse

• Synonym(s): P2X purinoceptor 7, ATP receptor, P2Z receptor, Purinergic receptor
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 14H7.1, monoclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_002553
• UniProt accession no.: Q99572
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... P2RX7(5027)

Description

General description

P2X purinoceptor 7 (UniProt: Q99572; also known as P2RX7, ATP receptor, P2Z receptor, Purinergic receptor) is encoded by the P2RX7 gene (Gene ID: 5027) in human. P2RX7 is a multi-pass membrane protein of the P2X receptor family that is widely expressed with highest levels reported in brain and immune tissues. Eight different isoforms of P2RX7 have been described that are generated via alternative splicing. Functional P2RX7 are shown to be organized as homomeric and as heteromeric trimers. They act as receptor for ATP and serve as a ligand-gated ion channel. They are also responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. They can function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. P2RX7 has two cytoplasmic domains (aa 1-25; 356-595); two transmembrane domains (aa 26-46; 335-355); and an extracellular domain (aa 47-334). P2RX7 can undergo phosphorylation that results in its inactivation. ADP-ribosylation at Arginine 125 is shown to be essential for its channel gating activity. Also, palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface.

Specificity

Clone 14H7.1 detects P2X purinoceptor 7 in human cells. It targets an epitope within 105 amino acids from the C-terminal half.

Immunogen

GST/His-tagged recombinant fragment corresponding to 105 amino acids from the C-terminal half of human P2X purinoceptor 7.

Application

Anti-P2RX7, clone 14H7.1, Cat. No. MABN1540, is a highly specific mouse monoclonal antibody that targets P2X purinoceptor 7 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected P2RX7 in human cerebellum and human tonsil tissue sections.

Research Category
Neuroscience

Quality

Evaluated by Western Blotting in human spleen tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected P2RX7 in 10 µg of human spleen tissue lysate.

Target description

~69 kDa observed; 68.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• wgk_germany: WGK 1