MABN1122

Anti-PSMA7, clone EPR5836, Rabbit Monoclonal Antibody

clone EPR5836, from rabbit

• Synonym(s): Proteasome subunit alpha type-7, Proteasome subunit RC6-1, Proteasome subunit XAPC7
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: unpurified
• antibody product type: primary antibodies
• clone: EPR5836, monoclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• UniProt accession no.: O14818
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PSMA7(5688)

Description

General description

PSMA (prostate specific membrane antigen) is a type II, integral membrane glycoprotein, composed of a 19 amino acid intracellular domain containing the N-terminus, a 24 amino acid transmembrane region, and a 707 amino acid extracellular C-terminal domain. The PSMA gene has been cloned and sequenced, and has been localized to chromosome 11q. PSMA is highly expressed in prostate secretory-acinar epithelium, in some benign extraprostatic epithelial cells from breast, duodenum, and kidney tissues, and in prostate cancer. Recently, evidence of limited and specific endothelial PSMA expression has been discovered: the neovasculature of a wide variety of malignant neoplasms (lung, colon, breast, etc.) demonstrates PSMA expression. This finding suggests that PSMA may be an effective target for monoclonal antibody-based anti-neovasculature therapy. PSMA has also been identified in the human nervous system. Based on activity, immunoreactivity, and mRNA sequence comparison with one form of NAALADase, a neuropeptide that may modulate glutaminergic transmission in the nervous system, PSMA was found to be indistinguishable from NAALADase derived from human cerebellar cell isolates. Therefore, the form of NAALADase also known as PSMA is expressed in brain, where it makes up a significant fraction of brain NAALADase activity.

Immunogen

Synthetic peptide corresponding to human PSMA7.

Application

Immunohistochemistry Analysis: A 1:250-500 dilution from a representative lot detected PSMA7 in human breast carcinoma tissue.

Produced in collaboration with Epitomics

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

This Anti-PSMA7, clone EPR5836, Rabbit Monoclonal Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of PSMA7.

Quality

Evaluated by Western Blotting in A549, MCF-7, HEK293, and HeLa cell lysates.

Western Blotting Analysis: A 1:1,000-10,000 dilution of this antibody detected PSMA7 in A549, MCF-7, HEK293, and HeLa cell lysates.

Target description

~27 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Unpurified

Rabbit monoclonal in buffer containing PBS with up to 0.1% sodium azide, 0.05% BSA, and 50% glycerol.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.