B-lymphocyte antigen CD19 (CD19), also known as Differentiation antigen CD19, is a transmembrane glycoprotein with two extracellular C2-type domains and is a critical signal transduction molecule that regulates B lymphocyte development, activation, and differentiation. CD19 expression is restricted to B cells, being absent from T cells, monocytes, and granulocytes. The CD19 antigen appears early during B cell maturation, probably at late pro-B cell stage around the time of Ig heavy chain rearrangement. It then persists during all stages of B cell maturation and is lost upon terminal differentiation to plasma cells.
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells.
Application
Anti-CD19 antibody (mouse), PE/Cy5, clone 6D5 is a highly specific rat monoclonal antibody & that targets CD19 & has been tested in Flow Cytometry.
Research Category Inflammation & Immunology
Research Sub Category Cytokines & Cytokine Receptors
Quality
The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody. Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of Cytiva and Carnegie Mellon University and made and sold under license from Cytiva.
Flow Cytometry Analysis: 0.25 μg of this antibody detected CD19 in one million C57BL/6 mouse splenocytes.
Target description
60 kDa calculated
Physical form
Affinity
Purified rat conjugate antibody in buffer containing PBS (pH 7.2) with up to 0.1% sodium azide.
Storage and Stability
The antibody solution should be stored undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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