MABF294

Anti-LAG3 Antibody, clone 8C7.1

culture supernatant, clone 8C7.1, from mouse

• Synonym(s): Lymphocyte activation gene 3 protein, LAG-3, Protein FDC, CD223, LAG3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: 8C7.1, monoclonal
• species reactivity: human
• technique(s): flow cytometry: suitable; western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_002277
• UniProt accession no.: P18627
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... LAG3(3902)

Description

General description

Lymphocyte activation gene 3 protein (UniProt P18627; also known as CD223, LAG-3, Protein FDC) is encoded by the LAG3 (also known as FDC) gene (Gene ID 3902) in human. LAG3 is a single-pass type I membrane protein that binds MHC class II molecules on antigen presenting cells with high affinity. LAG3 expression is induced after T cell activation and functions as a negative regulator of T cell function. LAG3 blockage is shown to augment T cell proliferation and cytokine production, and LAG3-deficient mice have a defect in the downregulation of T cell responses induced by staphylococcal enterotoxin B or by virus infection. In addition, LAG3 is expressed on activated regulatory T (Treg) cells at higher levels than on activated effector T cells. LAG3 blockage inhibits the suppressive activity of Treg cells. LAG3 is also upregulated on tumour-infiltrating lymphocytes (TILs), and LAG3 blockage can enhance anti-tumour T cell responses.

Immunogen

Mouse Fc-tagged recombinant human LAG3 extracellular fragment.

Application

Flow Cytometry Analysis: A 1:100 dilution of this antibody from a representative lot detected LAG3 in 1X10E6 Jurkat cells subjected to CD3/CD28 costimulation.

This Anti-LAG3 Antibody, clone 8C7.1 is validated for use in Western Blotting, Flow Cytometry for the detection of LAG3.

Quality

Evaluated by Western Blotting in NK-92c ell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LAG3 in 10 µg of NK-92 cell lysate.

Target description

~60 kDa observed

Physical form

Mouse monoclonal IgG2aκ antibody tissue culture supernatant with 0.05% sodium azide.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2