MABF241

Anti-PLGF Antibody, clone 216-17

clone 216-17, from mouse

• Synonym(s): Placenta growth factor, PlGF
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 216-17, monoclonal
• species reactivity: human
• technique(s): western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001193941
• UniProt accession no.: P49763
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PGF(5228)

Description

General description

Placenta growth factor (PGF, PIGF, PGFL, or PLGF) is a secreted protein primarily produced by placental trophoblast tissues but also expressed in other endothelial tissues and cancers. There are three isoforms, PGF-1, PGF-2, and PGF-3 (PIGF-1, PIGF-2, PIGF-3). PGF-2 is expressed up until week 8 in the placenta; PGF-1 and PGF-3 are continuously expressed by the placental tissues but only PGF-1 is found in colon and mammary carcinomas. PGF acts to stimulate angiogenesis, endothelial growth and migration. PGF is a powerful promoter of tumor growth and is upregulated in some cancers, and PGF is thought to aid in atherosclerotic lesions and neovascular growth surrounding the lesion. Also, PGF appears to aid aldosterone mediated atherosclerosis. Serum levels of PGF can be measured and in some cases used as a potential biomarker for disease or genetic defect. Recent research indicates that PGF levels are lower in mothers with Down syndrome fetuses, and when combined with additional tests PGF data helps lower the false positive detection rate in blood tests.

Immunogen

Recombinant protein corresponding to human PLGF.

Application

Detect Placenta growth factor using this mouse monoclonal antibody, Anti-PLGF Antibody & clone 216-17 validated for use in western blotting.

Research Category
Inflammation & Immunology

Research Sub Category
Growth Factors & Receptors

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PLGF in A431, HepG2, Jurkat, and PC3 cell lysate.

Quality

Evaluated by Western Blotting in HEK293T cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected PLGF in 10 µg of HEK293T cell lysate.

Target description

~19 kDa observed. The calculated molecular weight is 25 kDa (PLGF-3), 19 kDa (PLGF-1) and 17 kDa (PIGF-2). While the three isoforms are present in most placental tissues, PIGF-2 (17 kDa) is specific to early (8 week) placenta, whereas PlGF-1 (19 kDa) is only found in colon and mammary carcinomas. Ths protein can be N-glycosylated. PIGF can form homodimer or heterodimer with VEDF, which can be observed in human brain tissue lysates. Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Format: Purified

Protein A purified

Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable