MABF1982

Anti-EBOV GP Antibody, clone 5E6

clone 5E6, from mouse

• Synonym(s): Envelope glycoprotein, GP1, 2: GP
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 5E6, monoclonal
• species reactivity: virus, Ebola virus
• packaging: antibody small pack of 25 μL
• technique(s): ELISA: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; neutralization: suitable; western blot: suitable
• isotype: IgG2aλ
• NCBI accession no.: NP_066246
• UniProt accession no.: Q05320
• target post-translational modification: unmodified

Description

General description

Envelope glycoprotein (UniProt: Q05320; also known as GP1,2; GP) is encoded by the GP gene (Gene ID: 911829) in Zaire Ebola virus. Ebola virus (EBOV) is a filovirus that is shown to cause severe viral hemorrhagic fever with high lethality. It has a single stranded, negative-sense RNA genome that encodes seven viral structural proteins including nucleoprotein, virion proteins, and glycoprotein. The glycoprotein is the most important protein involved in pathogenesis. It is a type I transmembrane surface protein shown to be responsible for receptor binding, viral entry, and cellular tropism. The GP gene is reported to undergo transcriptional editing to give rise to several glycosylated proteins, including the structural protein GP1,2, and the secreted non-structural glycoprotein (sGP). The GP is synthesized as a 676-amino acid polyprotein with a signal peptide (aa 1-32). This polyprotein is cleaved by the furin to yield two disulfide linked subunits known as GP1 (aa 33-501) and GP2 (aa 502-676), which together form the GP1,2 heterodimer. The GP1 acts as the receptor-binding subunit and GP2 as the membrane fusion subunit. The GP1 subunit is expressed on the cell surface with the C-terminus oriented toward the aqueous environment, while the N-terminus is bound to GP2 via disulfide bonds. The GP1 subunit contains the heavily glycosylated mucin-like domain (aa 305-485), which is responsible for its cytotoxic function. The glycoprotein contains two coiled coil regions (aa 554-595 and 615-634) that play a role in its oligomerization and fusion activity. When administered before virus challenge, clone 5E6 can provide complete protection from cytopathic effects. (Ref.: Qiu, X., et al. (2011). Clin. Immunol. 141(2); 218-227).

Specificity

Clone 5E6 is a mouse monoclonal antibody that detects envelope glycoprotein from Zaire ebola virus.

Immunogen

Glycoprotein from Zaire Ebola virus, strain Mayinga, expressed in VSVdeltaG. Reverse genetics technique was used to create the recombinant virus VSVdeltaG/ZEBOV glycoprotein.

Application

Anti-EBOV GP, clone 5E6, Cat. No. MABF1982, is a mouse monoclonal antibody that detects envelope glycoprotein of Ebola virus and has been tested for use in ELISA, Immunocytochemistry, Neutraliziation, Immunoprecipitation, and Western Blotting.

Immunoprecipitation Analysis: A representative lot detected EBOV GP in Immunoprecipitation applications (Qiu, X., et. al. (2011). Clin Immunol. 141(2):218-27).

Neutralizing Analysis: A representative lot provided complete protection from cytopathic effects when administed prior to virus challenge. (Qiu, X., et. al. (2011). Clin Immunol. 141(2):218-27).

ELISA Analysis: A representative lot detected EBOV GP in ELISA applications (Qiu, X., et. al. (2011). Clin Immunol. 141(2):218-27).

Immunocytochemistry Analysis: A representative lot detected EBOV GP in Immunocytochemistry applications (Qiu, X., et. al. (2011). Clin Immunol. 141(2):218-27).

Western Blotting Analysis: A representative lot detected EBOV GP in Western Blotting applications (Qiu, X., et. al. (2011). Clin Immunol. 141(2):218-27).

Research Category
Inflammation & Immunology

Quality

Evaluated by ELISA with recombinant Ebola virus Glycoprotein minus the transmembrane region (EBOV rGPdeltaTM).

ELISA Analysis: Various dilutions of this antibody detected recombinant Ebola virus Glycoprotein minus the transmembrane region (EBOV rGPdeltaTM).

Target description

74.46 kDa calculated.

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in PBS without azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.