MABF187

Anti-KARS Antibody, clone 8G12C1

ascites fluid, clone 8G12C1, from mouse

• Synonym(s): Lysine-tRNA ligase, Lysyl-tRNA synthetase, LysRS
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 8G12C1, monoclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1
• UniProt accession no.: Q15046
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... KARS1(3735)

Description

General description

KARS, also known as Lysyl-tRNA synthetase (LysRS), is the enzyme that connects the lysine to its specific tRNA for use in protein translation. KARS is also a powerful inflammatory agent when released into extracellular matrix as one might expect. KARS is largely cytoplasmically localized or localized to internal membranes but isoforms are found in the mitochondria and nucleus as well. KARS became the focus of much attention with HIV-1 as KARS interacted with HIV-1’s GAG protein and facilitated the selective packaging of tRNA3(lys) nucleic acid which acted as the primer for viral reverse transcription and initiation of viral gene expression. There is also at least one genetic disease linked to KARS. It is the cause of Charcot-Marie-Tooth neuropathy a progressive peripheral nervous system disease characterized by progressive weakness and atrophy of the muscles and demyelination and deterioration the peripheral nerves and axons. EMD-Millipore’s Anti-KARS mouse monoclonal antibody has been tested in western blot on recombinant protein and in paraffin embedded immunohistochemistry on human cervical carcinoma tissue sections.

Immunogen

Purified recombinant fragment of human KARS expressed in E. Coli.

Application

Anti-KARS Antibody, clone 8G12C1 is a highly specific mouse monoclonal antibody, that targets Lysyl-tRNA synthetase & has been tested in western blotting & IHC.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected KARS in human cervical carcinoma and human tonsil tissues.

Optimal working dilutions must be determined by end user.

Research Category
Inflammation & Immunology

Research Sub Category
Immunological Signaling

Quality

Evaluated by Western Blotting in truncated Trx-KARS recombinant protein, truncated MBP-KARS and full length KARS transfected CHO-K1 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected KARS in truncated Trx-KARS recombinant protein, truncated MBP-KARS and full length KARS transfected CHO-K1 cell lysates.

Target description

~24, ~44, and ~50 kDa observed. This antibody recognizes truncated MBP-KARS at ~24 kDa, full length KARS at ~44 kDa, and transfected CHO-K1 cell lysate at ~50 kDa

Physical form

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Truncated Trx-KARS recombinant protein, truncated MBP-KARS and full length KARS transfected CHO-K1 cell lysates

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable