MABF1098
Anti-hnRNP A2B1 Antibody, clone 12B12.2
clone 12B12.2, from mouse
Synonym(s):
Heterogeneous nuclear ribonucleoproteins A2/B1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
12B12.2, monoclonal
species reactivity
human, mouse
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... HNRNPA2B1(3181)
mouse ... Hnrnpa2B1(53379)
General description
Heterogeneous nuclear ribonucleoproteins A2/B1 (UniProt: P22626; also known as hnRNP A2/B1) is encoded by the HNRNPA2B1 gene (Gene ID: 3181) in human. The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and associate with pre-mRNAs in the nucleus. These complexes are associated with pre-mRNA processing and other aspects of mRNA metabolism and transport. It contains 2 RNA recognition motifs. Through alternate splicing two isoforms are generated. Isoform 1 (B1) is localized in cytoplasmic mRNP granules containing untranslated mRNA and in exosomes. It is not found in the nucleolus. Isoform 2 (A2) is predominantly nucleoplasmic, however it is also found in the cytoplasm of cells in some tissues. The hnRNP particle arrangement on nascent hnRNA is non-random and sequence-dependent and serves to condense and stabilize the transcripts and minimize tangling and knotting. The hnRNP proteins are involved in transport of specific mRNAs to the cytoplasm in oligodendrocytes and neurons and they acts by specifically recognizing and binding the A2RE (21 nucleotide hnRNP A2 response element) or the A2RE11 (derivative 11 nucleotide oligonucleotide) sequence motifs present on some mRNAs, and promotes their transport to the cytoplasm. They are also reported to specifically bind single-stranded telomeric DNA sequences, thereby protecting telomeric DNA repeat against endonuclease digestion.
Specificity
Clone 12B12.2 specifically detects heterogeneous nuclear ribonucleoproteins A2/B1. it targets an epitope within 15 amino acids from the N-terminal region.
Immunogen
KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of human heterogeneous nuclear ribonucleoproteins A2/B1.
Application
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative detected hnRNP A2B1 in human tonsil, human small intestine, and human uterus tissues.
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected hnRNP A2B1 in 10 µg of NIH/3T3.
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected hnRNP A2B1 in 10 µg of NIH/3T3.
Research Category
Inflammation & Immunology
Inflammation & Immunology
This mouse monoclonal Anti-hnRNP A2B1, clone 12B12.2 Antibody, Cat. No. MABF1098 is tested in Immunohistochemistry (Paraffin) and in Western Blotting for the detection of hnRNP.
Quality
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected hnRNP A2B1 10 µg of HeLa cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected hnRNP A2B1 10 µg of HeLa cell lysate.
Target description
~37 kDa observed; 37.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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