Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Application
Anti-Histone H3.3 Antibody, clone 6C4A3 is an antibody against Histone H3.3 for use in western blotting.
Western Blotting Analysis: 0.1 µg/mL from a representative lot detected Histone H3.3 with recombinant H3.3 proteins. Additionally, 1 µg/mL from a representative lot detected Histone H3.3 in HeLa, COS1, NIH/3T3, NRK, and MDCK whole cell lysates (Prof. Taro Tachibana, Cell Engineering Corporation.).
Quality
Evaluated by Western Blotting in HeLa acid extract.
Western Blotting Analysis: 1 µg/mL of this antibody detected Histone H3.3 in 10 µg of HeLa acid extract.
Target description
~17 kDa observed
Physical form
Format: Purified
Not finding the right product?
Try our Product Selector Tool.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
The chromatin landscape and cellular metabolism both contribute to cell fate determination, but their interplay remains poorly understood. Using genome-wide siRNA screening, we have identified prohibitin (PHB) as an essential factor in self-renewal of human embryonic stem cells (hESCs). Mechanistically, PHB
Questions
Reviews
★★★★★ No rating value
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
