MABE923

Anti-Histone H3, clone 1B1-B2 Antibody

clone 1B1-B2, from mouse

• Synonym(s): Histone H3.3C, Histone H3.5
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1B1-B2, monoclonal
• species reactivity: yeast, mouse, human
• species reactivity (predicted by homology): vertebrates (based on 100% sequence homology)
• technique(s): ChIP: suitable; western blot: suitable
• isotype: IgG3κ
• NCBI accession no.: NP_001013721
• UniProt accession no.: Q6NXT2
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... H3C1(8350) ; mouse ... H3C1(360198)

Description

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Di- and tri-methylation of histone H3 are gene silencing marks, while removal of these marks by the demethylase genes Jmjd1a and Jmjd2c leads to gene activation.

Immunogen

Epitope: C-terminus

Linear peptide corresponding to the C-terminus of human Histone H3.

Application

Anti-Histone H3 antibody, clone 1B1-B2 is an antibody against Histone H3 for use in western blotting & ChIP.

Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated Histone H3 from mouse embyonic fibroblasts (MEF).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Histone H3 in 10 µg of HeLa cell lysate.

Target description

~15 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable