MABE894

Anti-Eri1/Exoribonuclease 1 Antibody, clone 5G8

clone 5G8, from rat

• Synonym(s): 3′-5′ exoribonuclease 1, 3′-5′ exonuclease ERI1, Eri-1 homolog, Histone mRNA 3′-exonuclease 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 5G8, monoclonal
• species reactivity: mouse
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_080343
• UniProt accession no.: Q7TMF2
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Eri1(67276)

Description

General description

The protein named 3’-5’ exoribonuclease 1 or 3′-5′ exonuclease ERI1, Eri-1 homolog, Histone mRNA 3′-end-specific exoribonuclease, Histone mRNA 3′-exonuclease 1, or Protein 3′hExo (HEXO), and encoded by the human gene ERI1/ 3’EXO,/THEX1 is an RNA exonuclease that binds to the 3′-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. Eri1 also can degrade 3’ overhangs on short interfering RNAs (siRNAs) suggestion a possible wider role in RNA interference, and finally Eri1 also processes other RNAs, including ribosomal RNAs and Eri1 is part of a pre-mRNA processing complex and can bind ribosomal subunits and assembled ribosomes too. Recent research also has identified Eri1 as a regulator of micro RNA and plays a role in miRNA homeostasis, lymphocyte development and antiviral functions. Eri1 is localized to the cytoplasm and nucleus, as well as, the nucleolus in normal cells.

Immunogen

Recombinant protein corresponding to mouse Eri1/Exoribonuclease 1.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

This Anti-Eri1/Exoribonuclease 1 Antibody, clone 5G8 is validated for use in western blotting & IP for the detection of Eri1/Exoribonuclease 1.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Eri1/Exoribonuclease 1 in 10 µg of mouse testis tissue lysate.

Western Blotting Analysis: A representative lot from an independent laboratory detected Eri1/Exoribonuclease 1 in MEF cell lysate (Hoefig, K. P., et al. (2012). Nat Struct Mol Biol. 20(1):73-81.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Eri1/Exoribonuclease 1 from MEF cell lysate (Hoefig, K. P., et al. (2012). Nat Struct Mol Biol. 20(1):73-81.).

Quality

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Eri1/Exoribonuclease 1 in 10 µg of NIH/3T3 cell lysate.

Target description

~40 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable