MABE431

Anti-PRMT1 Antibody, clone 7D2

clone 7D2, from rat

• Synonym(s): Protein arginine N-methyltransferase 1, Histone-arginine N-methyltransferase PRMT1, Interferon receptor 1-bound protein 4
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 7D2, monoclonal
• species reactivity: rat, mouse, human
• technique(s): western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_001527
• UniProt accession no.: Q99873
• shipped in: wet ice
• Gene Information: human ... PRMT1(3276)

Description

General description

The methylation of arginine is mediated by the Protein Arginine Methyl Transferase (PRMT) family of enzymes. These enzymes transfer the methyl group from S-adenosyl-Lmethionine to the guanidino nitrogen atoms of an arginine residue. Arginine methylation was found to be an important modification in signal transduction, transcription, RNA transport, and splicing. PRMT1, also known as HRMT1L2 and IR1B4, was isolated through its interaction with BTG1 and TIS21, proteins that are important in cell quiescence. PRMT1 is a 361 amino acid protein; its splicing variants differ at the N-terminus, giving rise to three isoforms of 343, 361, and 347 amino acids (variants 1, 2, and 3 respectively). Proteins involved in the metabolism of RNA are substrates for PRMT1, among them Sam68.

Immunogen

BSA-conjugated linear peptide corresponding to human PRMT1.

Application

Detect PRMT1 using this rat monoclonal antibody, Anti-PRMT1 Antibody & clone 7D2 validated for use in western blotting.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

Western Blotting Analysis: A representative lot from an independent laboratory detected PRMT1 in E. Coli containing untagged PRMT1, E.Coli extract containing His-tagged PRMT1, E.Coli extract containing GST- tagged PRMT1, and in DG75 whole cell extract (Gross, H., et al. (2012). PLoS ONE. 7(8):e42106.).

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PRMT1 in 10 µg of HEK293 cell lysate.

Target description

~ 42 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HEK293 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable