MABE350

Anti-phospho-RNAPII (Tyr1) Antibody, clone 3D12

clone 3D12, from rat

• Synonym(s): DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 3D12, monoclonal
• species reactivity: human
• technique(s): ChIP: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_000928
• UniProt accession no.: P24928
• shipped in: wet ice
• target post-translational modification: phosphorylation (pTyr1)

Description

General description

RNA polymerase II (RNAPII or Pol II) is a multi-subunit enzyme responsible for the transcription of transcription of DNA into RNA from protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. RNAPII can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

Immunogen

Ovalbumin-conjugated linear peptide corresponding to human RNAPII phosphorylated at Tyr1.

Application

Detect the phospho-RNAPII (Tyr1) protein using this Anti-phospho-RNAPII (Tyr1), clone 3D12 validated for use in WB, IP & ChIP.

Immunoprecipitation Analysis: A representative lot from an independent laboratory detected phospho-RNAPII (Tyr1) in proliferating yeast whole cell extract (Mayer, A., et al. (2012). Science. 336(6089):1723-1725.).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot from an independent laboratory immunoprecipitated phospho-RNAPII (Tyr1) in proliferating yeast whole cell extract (Mayer, A., et al. (2012). Science. 336(6089):1723-1725.).

Quality

Evaluated by Western Blot Analysis in untreated and lambda phosphatase treated HeLa cell lysates.

Western Blot Analysis: 2 µg/mL of this antibody detected phospho-RNAPII (Tyr1) in 10 µg of untreated HeLa cell lysate. (Lambda phosphatase treatment was performed during the western blot procedure.)

Target description

~220 kDa observed

Physical form

Format: Purified

Analysis Note

Control
Untreated and lambda phosphatase treated HeLa cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable