MABE246

Anti-dimethyl Histone H3 Antibody (Arg17), symmetric

clone 6E8-E11, from mouse

• Synonym(s): Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 6E8-E11, monoclonal
• species reactivity: human
• technique(s): dot blot: suitable; immunocytochemistry: suitable; western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_003520
• UniProt accession no.: P68431
• shipped in: wet ice
• target post-translational modification: dimethylation (Arg17)
• Gene Information: human ... HIST1H3F(8968)

Description

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Immunogen

KLH-conjugated linear peptide corresponding to human symmetric Histone H3 dimethylated at Arg17.

Application

Detect Histone H3 using this mouse monoclonal antibody, Anti-dimethyl Histone H3 Antibody (Arg17), symmetric validated for use in western blotting, Dot Blot & ICC.

Dot Blot Analysis: 1 µg/mL from a representative lot detected dimethyl Histone H3 (Arg17), symmetric in a panel of unmodified and modified Histones.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected dimethyl Histone H3 (Arg17), symmetric in A431 and HepG2 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Quality

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected dimethyl Histone H3 (Arg17), symmetric in 10 µg of HeLa nuclear extract.

Target description

~15 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable