MABE1531

Anti-Nuf2 Antibody, clone 28-37

clone 28-37, from mouse

• Synonym(s): Kinetochore protein Nuf2, Cell division cycle-associated protein 1, hNuf2, hNuf2R, hsNuf2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 28-37, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_113611
• UniProt accession no.: Q9BZD4
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... NUF2(83540)

Description

General description

Kinetochore protein Nuf2 (UniProt Q9BZD4; also known as Cell division cycle-associated protein 1, hNuf2, hNuf2R, hsNuf2) is encoded by the NUF2 (also known as CDCA1, NUF2R) gene (Gene ID 83540) in human. Nuf2 is a kinetochore complex component that plays an important role in stabilizing microtubule attachment. Nuf2 knockdown results in kinetochore attachment defects and eventual death of mitotic cells. Nuf2 binds and stabilizes CENP-E in the kinetochore, and both proteins are essential for proper chromosome segregation during mitosis. Nuf2 is also shown to complex with Hec1, and the Nuf2-Hec1 complex is essential for organizing microtubule attachment sites. Nuf2 upregulation is reported in various cancers, and Nuf2 knockdown results in significant cell growth delay and apoptosis among non-small cell lung, colorectal, gastric, and ovarian cancer cell lines.

Specificity

The specificity of clone 28-37 was confirmed by immunoblotting, immunofluorescence microscopy, and immunoprecipitation (Stucke, V.M., et al. (2004). Chromosoma. 113(1):1-15).

Immunogen

His-tagged full-length human Nuf2.

Application

Immunocytochemistry Analysis: A representative lot detected Nuf2 in Nocodazole-treated HeLa cells by immunofluorescence microscopy (Stucke, V.M., et al. (2004). Chromosoma. 113(1):1-15).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Nuf2 (Stucke, V.M., et al. (2004). Chromosoma. 113(1):1-15).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

This Anti-Nuf2 Antibody, clone 28-37 is validated for use in Western Blotting, Immunocytochemistry, Immunoprecipitation for the detection of Nuf2.

Quality

Evaluated by Western Blotting in HeLa cell acid extract.

Western Blotting Analysis: A 1:125 dilution of this antibody detected Nuf2 in 10 µg of HeLa cell acid extract.

Target description

~54 kDa observed. 54.30 kDa calculated.

Linkage

Replaces: 05-905

Physical form

Format: Purified

Protein G purified.

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1