MABE1133

Anti-TET3 Antibody, clone 23B9

clone 23B9, from rat

• Synonym(s): Methylcytosine dioxygenase TET3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 23B9, monoclonal
• species reactivity: mouse
• technique(s): ELISA: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_898961
• UniProt accession no.: Q8BG87
• shipped in: wet ice
• target post-translational modification: unmodified

Description

General description

Methylcytosine dioxygenase TET3 (EC 1.14.11.n2; UniProt Q8BG87) is encoded by the Tet3 gene (Gene ID 194388) in murine species. TET (ten-eleven translocation) proteins (TET1, TET2, and TET3) oxidize 5-methylcytosine to 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine, providing a means for active epigenetic DNA demethylation in mammals. TET proteins also modify genomic thymine residues to 5-hydroxyuracil. TET proteins depend on Fe(II) and 2-oxoglutarate as cofactors for activity, and 2-oxoglutarate is known to inhibit their activity, while ascorbate is shown to stimulate TET-mediated cytosine oxidation. In addition, post-translational modifications (PTMs), such as phosphorylation and O-GlcNAcylation, also play a role in regulating the activity of TET proteins. TET1 and TET2 are found highly expressed in mouse embryonic stem cells (mESCs) and TET3 is known to be upregulated in oocytes and oxidize the silenced paternal pronuclear DNA. High levels of TET proteins and genomic 5-hydroxymethylcytosine in neuronal tissues are also reported.

Specificity

Clone 23B9 epitope lies within the region between DNA-binding and 2-oxoglutarate-binding domains of murine TET3 sequence (UniProt Q8BG87-1). Clone 23B9 epitope region is not present in spliced isoform 2 (UniProt Q8BG87-2).

Immunogen

Epitope: Region between DNA-binding domain and 2-oxoglutarate-binding domain.

His-tagged recombinant internal fragment from the region between the DNA-binding domain and 2-oxoglutarate-binding domain of mouse TET3.

Application

Anti-TET3 Antibody, clone 23B9 is an antibody against TET3 for use in Immunocytochemistry, Immunoprecipitation, ELISA.

Immunoprecipitation Analysis: A representative lot immunoprecipitated GFP-tagged TET3 protein from lysates of HEK293T transfectants (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
ELISA Analysis: Clone 23B9 hybridoma culture supernatant was verified for its immunoreactivity against His-tagged murine TET3 by ELISA (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Developmental Signaling

Quality

Evaluated by Immunocytochemistry in mouse ES cells.

Immunocytochemistry Analysis: 1.0 µg/mL of this antibody detected TET3 in mouse ES cells.

Target description

180.4 calculated.

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable